Integrating cellular electron microscopy with multimodal data to explore biology across space and time.

McCafferty, Caitlyn L; Klumpe, Sven; Amaro, Rommie E; Kukulski, Wanda; Collinson, Lucy; Engel, Benjamin D (2024). Integrating cellular electron microscopy with multimodal data to explore biology across space and time. Cell, 187(3), pp. 563-584. Elsevier 10.1016/j.cell.2024.01.005

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Biology spans a continuum of length and time scales. Individual experimental methods only glimpse discrete pieces of this spectrum but can be combined to construct a more holistic view. In this Review, we detail the latest advancements in volume electron microscopy (vEM) and cryo-electron tomography (cryo-ET), which together can visualize biological complexity across scales from the organization of cells in large tissues to the molecular details inside native cellular environments. In addition, we discuss emerging methodologies for integrating three-dimensional electron microscopy (3DEM) imaging with multimodal data, including fluorescence microscopy, mass spectrometry, single-particle analysis, and AI-based structure prediction. This multifaceted approach fills gaps in the biological continuum, providing functional context, spatial organization, molecular identity, and native interactions. We conclude with a perspective on incorporating diverse data into computational simulations that further bridge and extend length scales while integrating the dimension of time.

Item Type:

Journal Article (Review Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Kukulski, Wanda

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1097-4172

Publisher:

Elsevier

Language:

English

Submitter:

Pubmed Import

Date Deposited:

05 Feb 2024 14:21

Last Modified:

05 Feb 2024 14:29

Publisher DOI:

10.1016/j.cell.2024.01.005

PubMed ID:

38306982

BORIS DOI:

10.48350/192419

URI:

https://boris.unibe.ch/id/eprint/192419

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