Waelti, Ernst R; Barton, Matthias (2006). Rapid endocytosis of copper-zinc superoxide dismutase into human endothelial cells: role for its vascular activity. Pharmacology, 78(4), pp. 198-201. Basel: Karger 10.1159/000096598
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Cytosolic CuZn-SOD (SOD1) is a dimeric, carbohydrate-free enzyme with a molecular weight of about 32 kDa and also circulates in human blood plasma. Due to its molecular mass it has been believed that the enzyme cannot penetrate the cell membrane. Here we report that rapid endocytosis of FITC-CuZn-SOD into human endothelial cells occurs within 5 min. Moreover, relaxation of rat aortic rings in response to CuZn-SOD is associated with a lag time of 45-60 s and only observed in the presence of intact endothelial cells. The results indicate acute and rapid endothelial cell endocytosis of CuZn-SOD, possibly via activation of a receptor-mediated pathway. Intracellular uptake via endocytosis may contribute to the vascular effects of CuZn-SOD, including vasodilation, and is likely to play a role in regulation of vascular tone and diseases such as atherosclerosis.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute of Pathology |
UniBE Contributor: |
Wälti, Ernst |
ISSN: |
0031-7012 |
ISBN: |
17077646 |
Publisher: |
Karger |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:49 |
Last Modified: |
21 Sep 2023 15:19 |
Publisher DOI: |
10.1159/000096598 |
PubMed ID: |
17077646 |
Web of Science ID: |
000241777800005 |
BORIS DOI: |
10.48350/20351 |
URI: |
https://boris.unibe.ch/id/eprint/20351 (FactScience: 3639) |
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