Detection of perforin and granzyme B mRNA expressing cells in lichen sclerosus

Hunger, Robert E; Brönnimann, Marcel; Kappeler, Andreas; Mueller, Christoph; Braathen, Lasse R; Yawalkar, Nikhil (2007). Detection of perforin and granzyme B mRNA expressing cells in lichen sclerosus. Experimental dermatology, 16(5), pp. 416-20. Oxford: Blackwell 10.1111/j.1600-0625.2007.00543.x

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Granzyme B and perforin messenger RNA (mRNA) expression has been shown to be a specific in vivo activation marker for cytotoxic cells. The aim of this study was to assess the contribution of cell-mediated cytotoxicity in the pathogenesis of lichen sclerosus. In situ hybridization and immunohistochemistry were performed on serial tissue sections of lesional skin biopsies and normal skin as control. Immunohistochemical staining showed that the cellular infiltrate of diseased skin consisted predominantly of T cells (CD3+) and some B cells (CD20+). Among T cells CD4+ and CD8+ cells were found in about equal numbers. In normal skin samples perforin and granzyme B mRNA expressing cells were only rarely found. In contrast, in biopsies from diseased skin a high percentage of infiltrating cells expressed mRNA for perforin and granzyme B. The perforin and granzyme B expressing cells were found in the dermal infiltrate and intraepidermally in close proximity to keratinocytes suggesting in situ activation of these cells. These findings provide evidence that cell-mediated cytotoxicity plays a significant role in tissue destruction in lichen sclerosus.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Dermatology 04 Faculty of Medicine > Service Sector > Institute of Pathology
UniBE Contributor:	Hunger, Robert, Kappeler, Andreas, Müller, Christoph (C), Yawalkar, Nikhil
ISSN:	0906-6705
ISBN:	17437484
Publisher:	Blackwell
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:53
Last Modified:	29 Mar 2023 23:32
Publisher DOI:	10.1111/j.1600-0625.2007.00543.x
PubMed ID:	17437484
Web of Science ID:	000245605500004
URI:	https://boris.unibe.ch/id/eprint/22641 (FactScience: 35698)