Characterisation of gp34, a GPI-anchored protein expressed by schizonts of Theileria parva and T. annulata

Xue, Gondga; von Schubert, Conrad; Hermann, Pascal; Peyer, Martina; Maushagen, Regina; Schmuckli-Maurer, Jacqueline; Bütikofer, Peter; Langsley, Gordon; Dobbelaere, Dirk, (2010). Characterisation of gp34, a GPI-anchored protein expressed by schizonts of Theileria parva and T. annulata. Molecular and biochemical parasitology, 172(2), pp. 113-20. Amsterdam: Elsevier 10.1016/j.molbiopara.2010.03.018

Full text not available from this repository.

Using bioinformatics tools, we searched the predicted Theileria annulata and T. parva proteomes for putative schizont surface proteins. This led to the identification of gp34, a GPI-anchored protein that is stage-specifically expressed by schizonts of both Theileria species and is downregulated upon induction of merogony. Transfection experiments in HeLa cells showed that the gp34 signal peptide and GPI anchor signal are also functional in higher eukaryotes. Epitope-tagged Tp-gp34, but not Ta-gp34, expressed in the cytosol of COS-7 cells was found to localise to the central spindle and midbody. Overexpression of Tp-gp34 and Ta-gp34 induced cytokinetic defects and resulted in accumulation of binucleated cells. These findings suggest that gp34 could contribute to important parasite-host interactions during host cell division.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Molecular Pathobiology 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
UniBE Contributor:	Von Schubert, Conrad, Schmuckli, Jacqueline, Bütikofer, Peter, Dobbelaere, Dirk,
ISSN:	0166-6851
Publisher:	Elsevier
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:12
Last Modified:	05 Dec 2022 14:01
Publisher DOI:	10.1016/j.molbiopara.2010.03.018
PubMed ID:	20381541
Web of Science ID:	000278907700007
URI:	https://boris.unibe.ch/id/eprint/2416 (FactScience: 204907)