Multiparameter flow cytometry characterization of MHC class I negative mouse bone marrow cells

Quarta, Mattia; Stroka, Deborah; Keogh, Adrian; Sidler, Daniel; Avital, Itzhak; Gloor, Beat; Muraca, Maurizio; Candinas, Daniel; Inderbitzin, Daniel (2008). Multiparameter flow cytometry characterization of MHC class I negative mouse bone marrow cells. Medical science monitor, 14(12), BR286-93. Warszawa: Medical Science International

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BACKGROUND: MHC-I down-regulation was described in foetal liver progenitors, and two different subsets of adult bone marrow derived stem cells. These cells, namely, MHC-I-/Thy1+ bone marrow derived liver stem cells (BMDLSC) and the multipotent adult progenitors (MAPC) differentiated into functioning hepatocytes. The aim of this paper was to characterize the MHC-I negative bone marrow compartment as it pertains to BMDLSC and MAPC. MATERIAL/METHODS: We performed multiparameter flow-cytometry analyses of the MHC-I negative compartment using hematopoietic (CD45, Ter119), and stem cell markers (Thy1.2, c-Kit, IL-3R, CD34) in adult mice. RESULTS: When analysing CD45 and Ter119 expression, the MHC-I negative bone marrow compartment divides into four sub-populations: 1. CD45-/Ter119+: 86.0+/-4.4%; 2. CD45+/Ter119+: 0.2+/-0.1%; 3. CD45+/Ter119-: 11.6+/-3.0%; 4. CD45-/Ter119-: 2.0+/-2.1%. Stem cells markers were only expressed on MHC-I negative/ CD45+/Ter119- cells. In vivo, MAPC (Ter119-/CD45- cells) are composed of MHC-I negative (24%) and MHC-I positive cells and do not express any of the stem cell markers tested. CONCLUSIONS: In conclusion, mouse BMDLSC and MAPC are two distinct stem cell populations. Down-regulation of MHC-I was the only common characteristic found between BMDLSC and MAPC suggesting that selection of MHC-I negative cells might represent an efficient strategy to enrich for bone marrow stem cells with liver developmental potential.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery 04 Faculty of Medicine > Service Sector > Institute of Clinical Pharmacology and Visceral Research [discontinued]
UniBE Contributor:	Stroka, Deborah, Keogh, Adrian, Sidler, Daniel (A), Gloor, Beat, Candinas, Daniel, Inderbitzin, Daniel
ISSN:	1234-1010
ISBN:	19043363
Publisher:	Medical Science International
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 15:05
Last Modified:	29 Mar 2023 23:33
PubMed ID:	19043363
Web of Science ID:	000262141000007
URI:	https://boris.unibe.ch/id/eprint/28457 (FactScience: 120857)