New respiratory enterovirus and recombinant rhinoviruses among circulating picornaviruses

Tapparel, Caroline; Junier, Thomas; Gerlach, Daniel; Van-Belle, Sandra; Turin, Lara; Cordey, Samuel; Mühlemann, Kathrin; Regamey, Nicolas; Aubert, John-David; Soccal, Paola M; Eigenmann, Philippe; Zdobnov, Evgeny; Kaiser, Laurent (2009). New respiratory enterovirus and recombinant rhinoviruses among circulating picornaviruses. Emerging infectious diseases, 15(5), pp. 719-726. Atlanta, Ga.: U.S. National Center for Infectious Diseases 10.3201/eid1505.081286

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Rhinoviruses and enteroviruses are leading causes of respiratory infections. To evaluate genotypic diversity and identify forces shaping picornavirus evolution, we screened persons with respiratory illnesses by using rhinovirus-specific or generic real-time PCR assays. We then sequenced the 5 untranslated region, capsid protein VP1, and protease precursor 3CD regions of virus-positive samples. Subsequent phylogenetic analysis identified the large genotypic diversity of rhinoviruses circulating in humans. We identified and completed the genome sequence of a new enterovirus genotype associated with respiratory symptoms and acute otitis media, confirming the close relationship between rhinoviruses and enteroviruses and the need to detect both viruses in respiratory specimens. Finally, we identified recombinants among circulating rhinoviruses and mapped their recombination sites, thereby demonstrating that rhinoviruses can recombine in their natural host. This study clarifies the diversity and explains the reasons for evolution of these viruses.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine

UniBE Contributor:

Mühlemann, Kathrin, Regamey, Nicolas

ISSN:

1080-6040

Publisher:

U.S. National Center for Infectious Diseases

Language:

English

Submitter:

Anette van Dorland

Date Deposited:

04 Oct 2013 15:12

Last Modified:

05 Dec 2022 14:22

Publisher DOI:

10.3201/eid1505.081286

PubMed ID:

19402957

Web of Science ID:

000265518800005

BORIS DOI:

10.7892/boris.31856

URI:

https://boris.unibe.ch/id/eprint/31856 (FactScience: 196625)

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