Ruepp, Marc-David; Aringhieri, Chiara; Vivarelli, Silvia; Cardinale, Stefano; Paro, Simona; Schümperli, Daniel; Barabino, Silvia M.L. (2009). Mammalian pre-mRNA 3' end processing factor CF I m 68 functions in mRNA export. Molecular biology of the cell, 20(24), pp. 5211-23. Bethesda, Md.: American Society for Cell Biology 10.1091/mbc.E09-05-0389
Full text not available from this repository.Export of mRNA from the nucleus is linked to proper processing and packaging into ribonucleoprotein complexes. Although several observations indicate a coupling between mRNA 3' end formation and export, it is not known how these two processes are mechanistically connected. Here, we show that a subunit of the mammalian pre-mRNA 3' end processing complex, CF I(m)68, stimulates mRNA export. CF I(m)68 shuttles between the nucleus and the cytoplasm in a transcription-dependent manner and interacts with the mRNA export receptor NXF1/TAP. Consistent with the idea that CF I(m)68 may act as a novel adaptor for NXF1/TAP, we show that CF I(m)68 promotes the export of a reporter mRNA as well as of endogenous mRNAs, whereas silencing by RNAi results in the accumulation of mRNAs in the nucleus. Moreover, CF I(m)68 associates with 80S ribosomes but not polysomes, suggesting that it is part of the mRNP that is remodeled in the cytoplasm during the initial stages of translation. These results reveal a novel function for the pre-mRNA 3' end processing factor CF I(m)68 in mRNA export.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology |
UniBE Contributor: |
Ruepp, Marc-David, Schümperli, Daniel |
ISSN: |
1059-1524 |
Publisher: |
American Society for Cell Biology |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 15:21 |
Last Modified: |
05 Dec 2022 14:25 |
Publisher DOI: |
10.1091/mbc.E09-05-0389 |
Web of Science ID: |
000272709800015 |
URI: |
https://boris.unibe.ch/id/eprint/36693 (FactScience: 205864) |
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