Rufener, Simone (24 January 2014). CTIF is involved in mRNA export in human cells (Unpublished). In: Swiss RNA Workshop 2014. Bern, Schweiz. 24.01.2014.
In eukaryotic cells translation initiation of messenger RNA (mRNA) transcripts can be initiated either by the cap-binding complex (CBC) consisting of CBP80 and CBP20, or by the eukaryotic translation initiation factor (eIF) 4E. Together with eIF4G and eIF4A, eIF4E forms the eIF4F-complex, which mediates translation initiation during the bulk of cellular protein synthesis [1,2]. Functionally analogous to eIF4G, the CBP80/20-dependent translation initiation factor (CTIF) has been reported to be part of the CBC-dependent translation initiation complex [3,4]. CTIF consists of a N-terminal CBP80-binding domain and a conserved C-terminal MIF4G domain [3]. This MIF4G domain has been shown to mediate the interaction between CTIF and different factors such as eIF3g and the stem-loop binding protein (SLBP) [4,5]. Here we show data indicating that CTIF, besides its function in translation initiation, is involved in mRNA translocation from the nucleus to the cytoplasm, possibly through a direct interaction with the nuclear export factor NFX1/TAP. Taken together our results suggest that CTIF can function as a platform that interacts with proteins involved in different steps of the mRNA metabolism.
[1] Haghighat A. and Sonenberg N. (1997) JBC 272:21677-21680
[2] Gross J.D. et al. (2003) Cell 115:739-750
[3] Kim K.M. et al. (2009) Genes Dev 23:2033-2045
[4] Choe J. et al. (2012) JBC 287:18500-18509
[5] Choe J. et al. (2013) NAR 41:1307-1318
Item Type: |
Conference or Workshop Item (Poster) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Rufener, Simone |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
Language: |
English |
Submitter: |
Christina Schüpbach |
Date Deposited: |
09 Feb 2015 08:40 |
Last Modified: |
05 Dec 2022 14:39 |
URI: |
https://boris.unibe.ch/id/eprint/62815 |
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