Optical properties of rabbit brain in the red and near-infrared: changes observed under in vivo, postmortem, frozen, and formalin-fixated conditions.

Pitzschke, Andreas; Lovisa, Blaise; Seydoux, Olivier; Hänggi, Matthias; Oertel, Markus Florian; Zellweger, Matthieu; Tardy, Yanik; Wagnières, Georges (2015). Optical properties of rabbit brain in the red and near-infrared: changes observed under in vivo, postmortem, frozen, and formalin-fixated conditions. Journal of biomedical optics, 20(2), p. 25006. SPIE International Society for Optical Engineering 10.1117/1.JBO.20.2.025006

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The outcome of light-based therapeutic approaches depends on light propagation in biological tissues, which is governed by their optical properties. The objective of this study was to quantify optical properties of brain tissue in vivo and postmortem and assess changes due to tissue handling postmortem. The study was carried out on eight female New Zealand white rabbits. The local fluence rate was measured in the VIS/NIR range in the brain in vivo, just postmortem, and after six weeks’ storage of the head at −20∘C or in 10% formaldehyde solution. Only minimal changes in the effective attenuation coefficient μeff were observed for two methods of sacrifice, exsanguination or injection of KCl. Under all tissue conditions, μeff decreased with increasing wavelengths. After long-term storage for six weeks at −20∘C, μeff decreased, on average, by 15 to 25% at all wavelengths, while it increased by 5 to 15% at all wavelengths after storage in formaldehyde. We demonstrated that μeff was not very sensitive to the method of animal sacrifice, that tissue freezing significantly altered tissue optical properties, and that formalin fixation might affect the tissue’s optical properties.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery 04 Faculty of Medicine > Department of Intensive Care, Emergency Medicine and Anaesthesiology (DINA) > Clinic of Intensive Care
UniBE Contributor:	Hänggi, Matthias, Oertel, Markus Florian
Subjects:	600 Technology > 610 Medicine & health
ISSN:	1083-3668
Publisher:	SPIE International Society for Optical Engineering
Language:	English
Submitter:	Alessandra Angelini
Date Deposited:	16 Mar 2015 11:50
Last Modified:	05 Dec 2022 14:42
Publisher DOI:	10.1117/1.JBO.20.2.025006
PubMed ID:	25706688
URI:	https://boris.unibe.ch/id/eprint/64397