Hollenstein, Marcel (2015). Generation of long, fully modified, and serum-resistant oligonucleotides by rolling circle amplification. Organic & biomolecular chemistry, 13(38), pp. 9820-9824. Royal Society of Chemistry 10.1039/C5OB01540E
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Rolling Circle Amplification (RCA) is an isothermal enzymatic method generating single-stranded DNA products consisting of concatemers containing multiple copies of the reverse complement of the circular template precursor. Little is known on the compatibility of modified nucleoside triphosphates (dN*TPs) with RCA, which would enable the synthesis of long, fully modified ssDNA sequences. Here, dNTPs modified at any position of the scaffold were shown to be compatible with rolling circle amplification, yielding long (>1 kb), and fully modified single-stranded DNA products. This methodology was applied for the generation of long, cytosine-rich synthetic mimics of telomeric DNA. The resulting modified oligo-nucleotides displayed an improved resistance to fetal bovine serum.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Hollenstein, Marcel |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISSN: |
1477-0520 |
Publisher: |
Royal Society of Chemistry |
Funders: |
[4] Swiss National Science Foundation |
Language: |
English |
Submitter: |
Marcel Hollenstein |
Date Deposited: |
03 Nov 2015 14:18 |
Last Modified: |
05 Dec 2022 14:49 |
Publisher DOI: |
10.1039/C5OB01540E |
BORIS DOI: |
10.7892/boris.72411 |
URI: |
https://boris.unibe.ch/id/eprint/72411 |
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