Human graft-derived mesenchymal stromal cells potently suppress alloreactive T-cell responses

de Mare-Bredemeijer, Emmy L D; Mancham, Shanta; Verstegen, Monique M A; de Ruiter, Petra E; van Gent, Rogier; O'Neill, David; Tilanus, Hugo W; Metselaar, Herold J; de Jonge, Jeroen; Kwekkeboom, Jaap; Hall, Sean; van der Laan, Luc J W (2015). Human graft-derived mesenchymal stromal cells potently suppress alloreactive T-cell responses. Stem cells and development, 24(12), pp. 1436-1447. Mary Ann Liebert 10.1089/scd.2014.0485

Text scd.2014.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (877kB)

After organ transplantation, recipient T cells contribute to graft rejection. Mesenchymal stromal cells from the bone marrow (BM-MSCs) are known to suppress allogeneic T-cell responses, suggesting a possible clinical application of MSCs in organ transplantation. Human liver grafts harbor resident populations of MSCs (L-MSCs). We aimed to determine the immunosuppressive effects of these graft-derived MSCs on allogeneic T-cell responses and to compare these with the effects of BM-MSCs. BM-MSCs were harvested from aspirates and L-MSCs from liver graft perfusates. We cultured them for 21 days and compared their suppressive effects with the effects of BM-MSCs on allogeneic T-cell responses. Proliferation, cytotoxic degranulation, and interferon-gamma production of alloreactive T cells were more potently suppressed by L-MSCs than BM-MSCs. Suppression was mediated by both cell-cell contact and secreted factors. In addition, L-MSCs showed ex vivo a higher expression of PD-L1 than BM-MSCs, which was associated with inhibition of T-cell proliferation and cytotoxic degranulation in vitro. Blocking PD-L1 partly abrogated the inhibition of cytotoxic degranulation by L-MSCs. In addition, blocking indoleamine 2,3-dioxygenase partly abrogated the inhibitive effects of L-MSCs, but not BM-MSCs, on T-cell proliferation. In conclusion, liver graft-derived MSC suppression of allogeneic T-cell responses is stronger than BM-MSCs, which may be related to in situ priming and mobilization from the graft. These graft-derived MSCs may therefore be relevant in transplantation by promoting allohyporesponsiveness.

Interest & Impact

Downloads

1 since deposited on 04 Apr 2016
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item