Fotiadis, Dimitrios; Müller, Daniel J (2013). High-resolution imaging of 2D outer membrane protein F (OmpF) crystals by atomic force microscopy. In: Schmidt-Krey, Ingeborg; Cheng, Yifan (eds.) Electron Crystallography of Soluble and Membrane Proteins. Methods in Molecular Biology: Vol. 955 (pp. 461-474). Humana Press 10.1007/978-1-62703-176-9_24
Full text not available from this repository.In this chapter the methodological bases are provided to achieve subnanometer resolution on two-dimensional (2D) membrane protein crystals by atomic force microscopy (AFM). This is outlined in detail with the example of AFM studies of the outer membrane protein F (OmpF) from the bacterium Escherichia coli (E. coli). We describe in detail the high-resolution imaging of 2D OmpF crystals in aqueous solution and under near-physiological conditions. The topographs of OmpF, and stylus effects and artifacts encountered when imaging by AFM are discussed.
Item Type: |
Book Section (Book Chapter) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
UniBE Contributor: |
Fotiadis, Dimitrios José |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
1064-3745 |
ISBN: |
978-1-62703-176-9 |
Series: |
Methods in Molecular Biology |
Publisher: |
Humana Press |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:23 |
Last Modified: |
05 Dec 2022 14:06 |
Publisher DOI: |
10.1007/978-1-62703-176-9_24 |
PubMed ID: |
23132075 |
URI: |
https://boris.unibe.ch/id/eprint/8148 (FactScience: 213635) |
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