Streptococcus pneumoniae proteins AmiA, AliA, and AliB bind peptides found in ribosomal proteins of other bacterial species

Nasher, Fauzy Negiub Ali; Heller, Manfred; Hathaway, Lucy Jane (2018). Streptococcus pneumoniae proteins AmiA, AliA, and AliB bind peptides found in ribosomal proteins of other bacterial species. Frontiers in Microbiology, 8(2688) Frontiers 10.3389/fmicb.2017.02688

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The nasopharynx is frequently colonized by both commensal and pathogenic bacteria including Streptococcus pneumoniae (pneumococcus). Pneumococcus is an important pathogen responsible for bacterial meningitis and community acquired pneumonia but is also commonly an asymptomatic colonizer of the nasopharynx. Understanding interactions between microbes may provide insights into pathogenesis. Here we investigated the ability of the three oligopeptide-binding proteins AmiA, AliA and AliB of an ATP-binding cassette transporter of pneumococcus to detect short peptides found in other bacterial species. We found three possible peptide ligands for AmiA and four each for AliA and AliB of which two for each protein matched ribosomal proteins of other bacterial species. Using synthetic peptides we confirmed the following binding: AmiA binds peptide AKTIKITQTR, matching 50S ribosomal subunit protein L30, AliA binds peptide FNEMQPIVDRQ, matching 30S ribosomal protein S20 and AliB binds peptide AIQSEKARKHN, matching 30S ribosomal protein S20, without excluding the possibility of binding of the other peptides. These Ami-AliA/AliB peptide ligands are found in multiple species in the class of Gammaproteobacteria which includes common colonizers of the nostrils and nasopharynx. Binding such peptides may enable pneumococcus to detect and respond to neighboring species in its environment and is a potential mechanism for interspecies communication and environmental surveillance.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Massenspektrometrie- und Proteomics-Labor

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Nasher, Fauzy Negiub Ali; Heller, Manfred and Hathaway, Lucy Jane

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1664-302X

Publisher:

Frontiers

Funders:

[UNSPECIFIED] Swiss National Science Foundation

Language:

English

Submitter:

Lucy Jane Hathaway

Date Deposited:

23 Feb 2018 13:43

Last Modified:

23 Feb 2018 13:43

Publisher DOI:

10.3389/fmicb.2017.02688

Uncontrolled Keywords:

Streptococcus pneumoniae, peptide, ABC transporter, Ami-AliA/AliB permease, interspecies communication

BORIS DOI:

10.7892/boris.109091

URI:

https://boris.unibe.ch/id/eprint/109091

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