Cytokeratin-based assessment of tumour budding in colorectal cancer: analysis in stage II patients and prospective diagnostic experience.

Koelzer, Viktor H; Assarzadegan, Naziheh; Dawson, Heather; Mitrovic, Bojana; Grin, Andrea; Messenger, David E; Kirsch, Richard; Riddell, Robert H; Lugli, Alessandro; Zlobec, Inti (2017). Cytokeratin-based assessment of tumour budding in colorectal cancer: analysis in stage II patients and prospective diagnostic experience. The journal of pathology: clinical research, 3(3), pp. 171-178. Wiley 10.1002/cjp2.73

[img]
Preview
Text
Koelzer_et_al-2017-The_Journal_of_Pathology__Clinical_Research.pdf - Published Version
Available under License Creative Commons: Attribution-Noncommercial (CC-BY-NC).

Download (410kB) | Preview

Tumour budding in colorectal cancer is an important prognostic factor. A recent consensus conference elaborated recommendations and key issues for future studies, among those the use of pan-cytokeratin stains, especially in stage II patients. We report the first prospective diagnostic experience using pan-cytokeratin for tumour budding assessment. Moreover, we evaluate tumour budding using pan-cytokeratin stains and disease-free survival (DFS) in stage II patients. To this end, tumour budding on pan-cytokeratin-stained sections was evaluated by counting the number of tumour buds in 10 high-power fields (0.238 mm2), then categorizing counts as low/high-grade at a cut-off of 10 buds, in two cohorts. Cohort 1: prospective setting with 236 unselected primary resected colorectal cancers analysed by 17 pathologists during diagnostic routine. Cohort 2: retrospective cohort of 150 stage II patients with information on DFS. In prospective analysis of cohort 1, tumour budding counts correlated with advanced pT, lymph node metastasis, lymphovascular invasion, perineural invasion (all p < 0.0001), and distant metastasis (p = 0.0128). In cohort 2, tumour budding was an independent predictor of worse DFS using counts [p = 0.037, HR (95% CI): 1.007 (1.0-1.014)] and the low-grade/high-grade scoring approach [p = 0.02; HR (95% CI): 3.04 (1.2-7.77), 90.7 versus 73%, respectively]. In conclusion, tumour budding assessed on pan-cytokeratin slides is feasible in a large pathology institute and leads to expected associations with clinicopathological features. Additionally, it is an independent predictor of poor prognosis in stage II patients and should be considered for risk stratification in future clinical studies.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology > Translational Research Unit
04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology > Autopsy

UniBE Contributor:

Koelzer, Viktor H; Dawson, Heather; Lugli, Alessandro and Zlobec, Inti

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2056-4538

Publisher:

Wiley

Language:

English

Submitter:

Inti Zlobec

Date Deposited:

01 Feb 2018 14:25

Last Modified:

01 Feb 2018 14:32

Publisher DOI:

10.1002/cjp2.73

PubMed ID:

28770101

Uncontrolled Keywords:

colorectal cancer cytokeratin pathology prognosis tumour budding

BORIS DOI:

10.7892/boris.110621

URI:

https://boris.unibe.ch/id/eprint/110621

Actions (login required)

Edit item Edit item
Provide Feedback