APOBEC3-mediated restriction of RNA virus replication

Milewska, Aleksandra; Kindler, Eveline Patricia; V'Kovski, Philip; Zeglen, S; Ochman, M; Thiel, Volker Earl; Rajfur, Z; Pyrc, K (2018). APOBEC3-mediated restriction of RNA virus replication. Scientific Reports, 8(1), p. 5960. Nature Publishing Group 10.1038/s41598-018-24448-2

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APOBEC3 family members are cytidine deaminases with roles in intrinsic responses to infection by retroviruses and retrotransposons, and in the control of other DNA viruses, such as herpesviruses, parvoviruses and hepatitis B virus. Although effects of APOBEC3 members on viral DNA have been demonstrated, it is not known whether they edit RNA genomes through cytidine deamination. Here, we investigated APOBEC3-mediated restriction of Coronaviridae. In experiments in vitro, three human APOBEC3 proteins (A3C, A3F and A3H) inhibited HCoV-NL63 infection and limited production of progeny virus, but did not cause hypermutation of the coronaviral genome. APOBEC3-mediated restriction was partially dependent on enzyme activity, and was reduced by the use of enzymatically inactive APOBEC3. Moreover, APOBEC3 proteins bound to the coronaviral nucleoprotein, and this interaction also affected viral replication. Although the precise molecular mechanism of deaminase-dependent inhibition of coronavirus replication remains elusive, our results further our understanding of APOBEC-mediated restriction of RNA virus infections.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Kindler, Eveline Patricia; V'Kovski, Philip and Thiel, Volker Earl

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

2045-2322

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Philip V'Kovski

Date Deposited:

09 May 2018 16:14

Last Modified:

15 May 2018 16:46

Publisher DOI:

10.1038/s41598-018-24448-2

PubMed ID:

29654310

BORIS DOI:

10.7892/boris.116455

URI:

https://boris.unibe.ch/id/eprint/116455

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