Is Penicillin Plus Gentamicin Synergistic Against Sessile Group B Streptococcal Isolates? An Study With an Experimental Model of Foreign-Body Infection.

Ruppen, Corinne; Mercier, Thomas; Grandgirard, Denis; Leib, Stephen; El Haj, Cristina; Murillo, Oscar; Decosterd, Laurent; Sendi, Parham (2018). Is Penicillin Plus Gentamicin Synergistic Against Sessile Group B Streptococcal Isolates? An Study With an Experimental Model of Foreign-Body Infection. Frontiers in Microbiology, 9, p. 919. Frontiers 10.3389/fmicb.2018.00919

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The rate of invasive group B (GBS) infections is steadily increasing, particularly in older persons and in adults with diabetes and other comorbidities. This population includes persons with a foreign body (e.g., who have undergone arthroplasty). In a rat tissue cage model, we evaluated the efficacy of adjunctive gentamicin (GEN) administered systemically (5 mg/kg body weight) every 24 h, or locally (12.5 mg/L tissue cage concentration) every 24 or 72 h, in combination with penicillin (PEN) administered systemically (250,000 IU/kg body weight three times per day). The efficacy was evaluated on two different sessile forms of GBS: transition (i.e., in between planktonic and biofilm) and biofilm. After 3 days of treatment, the mean bacterial load reduction of transition-form GBS was greater in all PEN-GEN combination groups than in the PEN monotherapy group ( ≤ 0.03). The 6-day regimen decreased the bacterial load significantly in comparison to the 3-day regimen, irrespective of growth form and adjunctive GEN ( < 0.01). After 6 days of treatment, the mean reduction in transition-form GBS was greater with PEN plus GEN administered locally every 24 h than with PEN monotherapy ( = 0.03). These results were not confirmed with biofilm GBS. The difference in mean bacterial load reduction between all PEN-GEN and PEN monotherapy groups was <100 CFU/mL. Hence, synergy criteria were not fulfilled. Adjunctive systemic GEN consists of potential side effects and showed poor efficacy in this study. Combining systemic PEN and local GEN has a potential application in the treatment of streptococcal implant-associated infections.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Ruppen, Corinne; Grandgirard, Denis; Leib, Stephen and Sendi, Parham

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1664-302X

Publisher:

Frontiers

Funders:

[UNSPECIFIED] Velux Stiftung (724 to PS),

Language:

English

Submitter:

Stephen Leib

Date Deposited:

29 Aug 2018 10:36

Last Modified:

05 Nov 2019 07:33

Publisher DOI:

10.3389/fmicb.2018.00919

PubMed ID:

29867830

Uncontrolled Keywords:

Streptococcus agalactiae biofilms foreign bodies gentamicin penicillins synergism

BORIS DOI:

10.7892/boris.119624

URI:

https://boris.unibe.ch/id/eprint/119624

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