The volatile molecular profiles of seven Streptococcus pneumoniae serotypes

Mellors, Theodore R; Rees, Christiaan A; Franchina, Flavio A; Burklund, Alison; Patel, Chaya; Hathaway, Lucy Jane; Hill, Jane E (2018). The volatile molecular profiles of seven Streptococcus pneumoniae serotypes. Journal of chromatography. B - analytical technologies in the biomedical and life sciences, 1096, pp. 208-213. Elsevier 10.1016/j.jchromb.2018.08.032

Text 1-s2.0-S1570023218302642-main.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (886kB) | Request a copy

In this study, the volatile molecule profile of Streptococcus pneumoniae serotypes was evaluated using solid phase microextraction (SPME) and two dimensional gas chromatography time-of-flight mass spectrometry (GC × GC-TOFMS). Here, seven serotypes (6B, 14, 15, 18C, 19F, 9V, and 23F) were analyzed in an isogenic background. We identified 13 core molecules associated with all seven serotypes, and seven molecules that were differentially produced between serotypes. Serotype 14 was found to have the most distinct volatile profile, and could be discriminated from the other six serotypes in aggregate with an area under the curve (AUC) of 89%. This study suggests that molecules from S. pneumoniae culture headspace show potential for rapid serotype identification.

Interest & Impact

Downloads

2 since deposited on 02 Oct 2018
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item