The volatile molecular profiles of seven Streptococcus pneumoniae serotypes

Mellors, Theodore R; Rees, Christiaan A; Franchina, Flavio A; Burklund, Alison; Patel, Chaya; Hathaway, Lucy Jane; Hill, Jane E (2018). The volatile molecular profiles of seven Streptococcus pneumoniae serotypes. Journal of chromatography. B - analytical technologies in the biomedical and life sciences, 1096, pp. 208-213. Elsevier 10.1016/j.jchromb.2018.08.032

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In this study, the volatile molecule profile of Streptococcus pneumoniae serotypes was evaluated using solid phase microextraction (SPME) and two dimensional gas chromatography time-of-flight mass spectrometry (GC × GC-TOFMS). Here, seven serotypes (6B, 14, 15, 18C, 19F, 9V, and 23F) were analyzed in an isogenic background. We identified 13 core molecules associated with all seven serotypes, and seven molecules that were differentially produced between serotypes. Serotype 14 was found to have the most distinct volatile profile, and could be discriminated from the other six serotypes in aggregate with an area under the curve (AUC) of 89%. This study suggests that molecules from S. pneumoniae culture headspace show potential for rapid serotype identification.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Bacteriology

UniBE Contributor:

Hathaway, Lucy Jane

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1570-0232

Publisher:

Elsevier

Language:

English

Submitter:

Lucy Jane Hathaway

Date Deposited:

02 Oct 2018 14:31

Last Modified:

24 Oct 2019 00:48

Publisher DOI:

10.1016/j.jchromb.2018.08.032

PubMed ID:

30179753

BORIS DOI:

10.7892/boris.120267

URI:

https://boris.unibe.ch/id/eprint/120267

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