Placental secretion of apolipoprotein A1 and E: the anti-atherogenic impact of the placenta.

Melhem, Hassan; Kallol, Sampada; Huang, Xiao; Lüthi, Michael; Ontsouka, Edgar; Keogh, Adrian; Stroka, Deborah; Thormann, Wolfgang; Schneider, Henning; Albrecht, Christiane (2019). Placental secretion of apolipoprotein A1 and E: the anti-atherogenic impact of the placenta. Scientific Reports, 9(1), p. 6225. Nature Publishing Group 10.1038/s41598-019-42522-1

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High levels of atherogenic lipids in pregnancy are associated with health complications for the mother, the fetus and the newborn. As endocrine secretory tissue, the human placenta releases apolipoproteins (apos), particularly apoA1 and apoE. However, the magnitude and the directionality of the apo secretions remain unknown. We aimed to 1) determine the amount and orientation (apical-maternal versus basal-fetal) of placentally secreted apoA1 and apoE using human perfused placenta and primary trophoblast cell (PTC) culture, 2) compare apoA1 and apoE secretions of PTC with that of hepatocytes and 3) associate the obtained results with human blood levels by determining apoA1 and apoE concentrations in maternal and fetal serum samples. In perfused placenta and serum samples, apoA1 and apoE concentrations were significantly higher at the maternal compared to the fetal side. For apoE a similar trend was found in PTC. For apoA1, the secretion to the apical side declined over time while release to the basal side was stable resulting in significantly different apoA1 concentrations between both sides. Unexpectedly, PTC secreted significantly higher amounts of apoA1 and apoE compared to hepatocytes. Our data indicate that the placenta may play an important role in maternal and fetal cholesterol homeostasis via secretion of anti-atherogenic apos.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Faculty Institutions > NCCR TransCure
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Laboratory for Clinical Pharmacology

UniBE Contributor:

Melhem, Hassan; Kallol, Sampada Arvindrao; Huang, Xiao; Lüthi, Michael; Ontsouka, Edgar; Keogh, Adrian; Keogh-Stroka, Deborah M.; Thormann, Wolfgang; Schneider, Henning and Albrecht, Christiane

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2045-2322

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Barbara Järmann-Bangerter

Date Deposited:

26 Sep 2019 09:15

Last Modified:

24 Oct 2019 07:17

Publisher DOI:

10.1038/s41598-019-42522-1

PubMed ID:

30996342

BORIS DOI:

10.7892/boris.133477

URI:

https://boris.unibe.ch/id/eprint/133477

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