The Dual Prey-Inactivation Strategy of Spiders—In-Depth Venomic Analysis of Cupiennius salei

Kuhn-Nentwig, Lucia; Langenegger, Nicolas; Heller, Manfred; Koua, Dominique; Nentwig, Wolfgang (2019). The Dual Prey-Inactivation Strategy of Spiders—In-Depth Venomic Analysis of Cupiennius salei. Toxins, 11(3) MDPI 10.3390/toxins11030167

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Most knowledge of spider venom concerns neurotoxins acting on ion channels, whereasproteins and their significance for the envenomation process are neglected. The here presentedcomprehensive analysis of the venom gland transcriptome and proteome ofCupiennius saleifocusses on proteins and cysteine-containing peptides and offers new insight into the structureand function of spider venom, here described as the dual prey-inactivation strategy. After venominjection, many enzymes and proteins, dominated byα-amylase, angiotensin-converting enzyme,and cysteine-rich secretory proteins, interact with main metabolic pathways, leading to a majordisturbance of the cellular homeostasis. Hyaluronidase and cytolytic peptides destroy tissue andmembranes, thus supporting the spread of other venom compounds. We detected 81 transcripts ofneurotoxins from 13 peptide families, whereof two families comprise 93.7% of all cysteine-containingpeptides. This raises the question of the importance of the other low-expressed peptide families.The identification of a venom gland-specific defensin-like peptide and an aga-toxin-like peptide inthe hemocytes offers an important clue on the recruitment and neofunctionalization of body proteinsand peptides as the origin of toxins.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Massenspektrometrie- und Proteomics-Labor
08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Community Ecology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Kuhn-Nentwig, Lucia Gerda; Langenegger, Nicolas; Heller, Manfred; Koua, Dominique Kadio and Nentwig, Wolfgang

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)

ISSN:

2072-6651

Publisher:

MDPI

Language:

English

Submitter:

Nicolas Langenegger

Date Deposited:

18 Nov 2019 14:05

Last Modified:

18 Nov 2019 14:05

Publisher DOI:

10.3390/toxins11030167

PubMed ID:

30893800

BORIS DOI:

10.7892/boris.135072

URI:

https://boris.unibe.ch/id/eprint/135072

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