Spider Venom: Components, Modes of Action, and Novel Strategies in Transcriptomic and Proteomic Analyses

Langenegger, Nicolas; Nentwig, Wolfgang; Kuhn-Nentwig, Lucia (2019). Spider Venom: Components, Modes of Action, and Novel Strategies in Transcriptomic and Proteomic Analyses. Toxins, 11(10) MDPI 10.3390/toxins11100611

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This review gives an overview on the development of research on spider venoms with a focus on structure and function of venom components and techniques of analysis. Major venom component groups are small molecular mass compounds, antimicrobial (also called cytolytic, or cationic) peptides (only in some spider families), cysteine-rich (neurotoxic) peptides, and enzymes and proteins. Cysteine-rich peptides are reviewed with respect to various structural motifs, their targets (ion channels, membrane receptors), nomenclature, and molecular binding. We further describe the latest findings concerning the maturation of antimicrobial, and cysteine-rich peptides that are in most known cases expressed as propeptide-containing precursors. Today, venom research, increasingly employs transcriptomic and mass spectrometric techniques. Pros and cons of venom gland transcriptome analysis with Sanger, 454, and Illumina sequencing are discussed and an overview on so far published transcriptome studies is given. In this respect, we also discuss the only recently described cross contamination arising from multiplexing in Illumina sequencing and its possible impacts on venom studies. High throughput mass spectrometric analysis of venom proteomes (bottom-up, top-down) are reviewed.

Item Type:

Journal Article (Review Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE)
08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Community Ecology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Langenegger, Nicolas; Nentwig, Wolfgang and Kuhn-Nentwig, Lucia Gerda

Subjects:

500 Science > 540 Chemistry
500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)

ISSN:

2072-6651

Publisher:

MDPI

Language:

English

Submitter:

Nicolas Langenegger

Date Deposited:

18 Nov 2019 13:49

Last Modified:

18 Nov 2019 13:49

Publisher DOI:

10.3390/toxins11100611

PubMed ID:

31652611

BORIS DOI:

10.7892/boris.135074

URI:

https://boris.unibe.ch/id/eprint/135074

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