Resistance formation to nitro drugs in Giardia lamblia: No common markers identified by comparative proteomics.

Müller, Joachim; Braga, Sophie; Heller, Manfred; Müller, Norbert (2019). Resistance formation to nitro drugs in Giardia lamblia: No common markers identified by comparative proteomics. International journal for parasitology. Drugs and drug resistance, 9, pp. 112-119. Elsevier 10.1016/j.ijpddr.2019.03.002

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In order to elucidate the question whether resistance to nitro drugs in G. lamblia is due to common resistance markers, trophozoites of three resistant G. lamblia strains, namely C4, 1062ID10, and 713M3 were grown in the presence of the two nitro drugs metronidazole and nitazoxanide and compared to their corresponding wild-types WBC6, 106, and 713 by mass spectometry shotgun analysis of their proteomes. Depending on the strain and the nitro drug, more than 200 to 500 differentially expressed proteins were identified, but there were no common patterns across strains and drugs. All resistant strains underwent antigenic variation with distinct surface antigens like variant surface proteins or cysteine rich proteins depending on strain and nitro compound. A closer look on enzymes involved in nitroreduction and detoxification of nitro radicals, NO or O2 suggested the existence of distinct strategies for each drug and each strain. Therefore, we conclude that resistance to nitro drugs in G. lamblia is not correlated with a specific pattern of differentially expressed proteins and therefore seems not to be the result of a directed process.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Massenspektrometrie- und Proteomics-Labor
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Müller, Joachim; Braga, Sophie Marie-Pierre; Heller, Manfred and Müller, Norbert

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

2211-3207

Publisher:

Elsevier

Language:

English

Submitter:

Marla Rittiner

Date Deposited:

17 Dec 2019 10:53

Last Modified:

17 Dec 2019 10:53

Publisher DOI:

10.1016/j.ijpddr.2019.03.002

PubMed ID:

30889439

Uncontrolled Keywords:

Adaptation Drug susceptibility Resistance Tolerance

BORIS DOI:

10.7892/boris.136303

URI:

https://boris.unibe.ch/id/eprint/136303

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