Pneumococcal serotype determines growth and capsule size in human cerebrospinal fluid

Müller, Annelies; Salmen, Anke; Aebi, Susanne; de Gouveia, Linda; von Gottberg, Anne; Hathaway, Lucy J. (2020). Pneumococcal serotype determines growth and capsule size in human cerebrospinal fluid. BMC microbiology, 20(1), p. 16. BioMed Central 10.1186/s12866-020-1700-7

[img]
Preview
Text
Müller et al 2020.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (892kB) | Preview

Background: The polysaccharide capsule is a major virulence factor of S. pneumoniae in diseases such as meningitis. While some capsular serotypes are more often found in invasive disease, high case fatality rates are associated with those serotypes more commonly found in asymptomatic colonization. We tested whether growth patterns and capsule size in human cerebrospinal fluid depends on serotype using a clinical isolate of S. pneumoniae and its capsule switch mutants.
Results: We found that the growth pattern differed markedly from that in culture medium by lacking the exponential and lysis phases. Growth in human cerebrospinal fluid was reduced when strains lost their capsules. When a capsule was present, growth was serotype-specific: high carriage serotypes (6B, 9V, 19F and 23F) grew better than low carriage serotypes (7F, 14, 15B/C and 18C). Growth correlated with the case-fatality rates of serotypes reported in the literature. Capsule size in human cerebrospinal fluid also depended on serotype.
Conclusions: We propose that serotype-specific differences in disease severity observed in meningitis patients may, at least in part, be explained by differences in growth and capsule size in human cerebrospinal fluid. This information could be useful to guide future vaccine design.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Müller, Annelies Kathrin; Salmen, Anke; Aebi, Susanne and Hathaway, Lucy Jane

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

1471-2180

Publisher:

BioMed Central

Funders:

[UNSPECIFIED] Swiss National Science Foundation

Language:

English

Submitter:

Lucy Jane Hathaway

Date Deposited:

05 Feb 2020 11:31

Last Modified:

16 Sep 2020 05:58

Publisher DOI:

10.1186/s12866-020-1700-7

PubMed ID:

31959125

Uncontrolled Keywords:

Streptococcus pneumoniae, human cerebrospinal fluid, serotype, capsule, growth, case fatality rate

BORIS DOI:

10.7892/boris.139460

URI:

https://boris.unibe.ch/id/eprint/139460

Actions (login required)

Edit item Edit item
Provide Feedback