Screen-Printed Glucose Sensors Modified with Cellulose Nanocrystals (CNCs) for Cell Culture Monitoring

Tang, Ye; Petropoulos, Konstantinos; Kurth, Felix; Hui, Gao; Migliorelli, Davide; Guenat, Olivier; Generelli, Silvia (2020). Screen-Printed Glucose Sensors Modified with Cellulose Nanocrystals (CNCs) for Cell Culture Monitoring. Biosensors, 10(9), p. 125. MDPI 10.3390/bios10090125

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Glucose sensors are potentially useful tools for monitoring the glucose concentration in cell culture medium. Here, we present a new, low-cost, and reproducible sensor based on a cellulose-based material, 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) oxidized-cellulose nanocrystals (CNCs). This novel biocompatible and inert nanomaterial is employed as a polymeric matrix to immobilize and stabilize glucose oxidase in the fabrication of a reproducible, operationally stable, highly selective, cost-effective, screen-printed glucose sensor. The sensors have a linear range of 0.1–2 mM (R2 = 0.999) and a sensitivity of 5.7 ± 0.3 μA cm−2∙mM−1. The limit of detection is 0.004
mM, and the limit of quantification is 0.015 mM. The sensor maintains 92.3% of the initial current response after 30 consecutive measurements in a 1 mM standard glucose solution, and has a shelf life of 1 month while maintaining high selectivity. We demonstrate the practical application of the sensor by monitoring the glucose consumption of a fibroblast cell culture over the course of several
days.

Item Type:

Journal Article (Original Article)

Division/Institute:

10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Organs-on Chip Technologies

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Tang, Ye and Guenat, Olivier Thierry

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
500 Science > 540 Chemistry
600 Technology > 620 Engineering

ISSN:

2079-6374

Publisher:

MDPI

Language:

English

Submitter:

Olivier Thierry Guenat

Date Deposited:

14 Sep 2020 17:15

Last Modified:

29 Sep 2020 20:53

Publisher DOI:

10.3390/bios10090125

BORIS DOI:

10.7892/boris.146505

URI:

https://boris.unibe.ch/id/eprint/146505

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