Innovative high-resolution microCT imaging of animal brain vasculature

Hlushchuk, Ruslan; Haberthür, David; Soukup, Petr; Barré, Sebastien F.; Khoma, Oleksiy-Zakhar; Schittny, Johannes; Haghayegh Jahromi, Neda; Bouchet, Audrey; Engelhardt, Britta; Djonov, Valentin Georgiev (2020). Innovative high-resolution microCT imaging of animal brain vasculature. Brain structure & function, 225(9), pp. 2885-2895. Springer 10.1007/s00429-020-02158-8

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Analysis of the angioarchitecture and quantification of the conduit vessels and microvasculature is of paramount importance for understanding the physiological and pathological processes within the central nervous system (CNS). Most of the available in vivo imaging methods lack penetration depth and/or resolution. Some ex vivo methods may provide better resolution, but are mainly destructive, as they are designed for imaging the CNS tissues after their removal from the skull or vertebral column. The removal procedure inevitably alters the in situ relations of the investigated structures and damages the dura mater and leptomeninges. µAngiofil, a polymer-based contrast agent, permits a qualitatively novel postmortem microangio-computed tomography (microangioCT) approach with excellent resolution and, therefore, visualization of the smallest brain capillaries. The datasets obtained empower a rather straightforward quantitative analysis of the vascular tree, including the microvasculature. The µAngiofil has an excellent filling capacity as well as a radio-opacity higher than the one of bone tissue, which allows imaging the cerebral microvasculature even within the intact skull or vertebral column. This permits in situ visualization and thus investigation of the dura mater and leptomeningeal layers as well as their blood supply in their original geometry. Moreover, the methodology introduced here permits correlative approaches, i.e., microangioCT followed by classical histology, immunohistochemistry and even electron microscopy. The experimental approach presented here makes use of common desktop microCT scanners, rendering it a promising everyday tool for the evaluation of the (micro)vasculature of the central nervous system in preclinical and basic research.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Hlushchuk, Ruslan; Haberthür, David; Khoma, Oleksiy-Zakhar; Schittny, Johannes; Haghayegh Jahromi, Neda; Bouchet, Audrey Michele; Engelhardt, Britta and Djonov, Valentin Georgiev

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1863-2661

Publisher:

Springer

Funders:

[4] Swiss National Science Foundation ; [UNSPECIFIED] Fidelity Bermuda Foundation

Language:

English

Submitter:

David Christian Haberthür

Date Deposited:

04 Dec 2020 10:26

Last Modified:

15 Mar 2021 22:51

Publisher DOI:

10.1007/s00429-020-02158-8

PubMed ID:

33128675

BORIS DOI:

10.7892/boris.147724

URI:

https://boris.unibe.ch/id/eprint/147724

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