Respiratory tissue-associated commensal bacteria offer therapeutic potential against pneumococcal colonization.

Yildiz, Soner; Pereira Bonifacio Lopes, João P; Bergé, Matthieu; González-Ruiz, Víctor; Baud, Damian; Kloehn, Joachim; Boal-Carvalho, Inês; Schaeren, Olivier P; Schotsaert, Michael; Hathaway, Lucy J; Rudaz, Serge; Viollier, Patrick H; Hapfelmeier, Siegfried; Francois, Patrice; Schmolke, Mirco (2020). Respiratory tissue-associated commensal bacteria offer therapeutic potential against pneumococcal colonization. eLife, 9 eLife Sciences Publications 10.7554/eLife.53581

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Under eubiotic conditions commensal microbes are known to provide a competitive barrier against invading bacterial pathogens in the intestinal tract, on the skin or on the vaginal mucosa. Here, we evaluate the role of lung microbiota in Pneumococcus colonization of the lungs. In eubiosis, the lungs of mice were dominantly colonized by Lactobacillus murinus. Differential analysis of 16S rRNA gene sequencing or L. murinus-specific qPCR of DNA from total organ homogenates vs.broncho alveolar lavages implicated tight association of these bacteria with the host tissue. Pure L. murinus conditioned culture medium inhibited growth and reduced the extension of pneumococcal chains. Growth inhibition in vitro was likely dependent on L. murinus-produced lactic acid, since pH neutralization of the conditioned medium aborted the antibacterial effect. Finally, we demonstrate that L. murinus provides a barrier against pneumococcal colonization in a respiratory dysbiosis model after an influenza A virus infection, when added therapeutically.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Schären, Olivier Pascal; Hathaway, Lucy Jane and Hapfelmeier, Siegfried Hektor

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2050-084X

Publisher:

eLife Sciences Publications

Language:

English

Submitter:

Siegfried Hektor Hapfelmeier-Balmer

Date Deposited:

31 Dec 2020 12:31

Last Modified:

31 Dec 2020 12:31

Publisher DOI:

10.7554/eLife.53581

PubMed ID:

33287959

Uncontrolled Keywords:

Influenza A virus Streptococcus pneumoniae colonization resistance infectious disease lactobacillus murinus lung microbiome microbiology mouse probiotics virus

BORIS DOI:

10.48350/150215

URI:

https://boris.unibe.ch/id/eprint/150215

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