Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures.

Gultom, Mitra; Laloli, Laura; Dijkman, Ronald (2020). Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures. In: Maier, Helena J.; Bickerton, Erica (eds.) Coronaviruses. Methods and Protocols. Methods in Molecular Biology: Vol. 2203 (pp. 119-134). Humana, New York, NY 10.1007/978-1-0716-0900-2_10

Full text not available from this repository.

Well-differentiated primary airway epithelial cell (AEC) cultures have been widely used for the characterization of several human respiratory viruses including coronaviruses. In recent years, there has been an increase in interest toward animal AEC cultures and their application to characterize veterinary viruses with zoonotic potential, as well as studying host-pathogen interactions in animal reservoir host species. In this chapter, we provide a revised and improved protocol for the isolation and establishment of well-differentiated AEC cultures from diverse mammalian species and the use of the cultures for the characterization of veterinary coronavirus. We also describe immunohistochemistry protocols with validated antibodies for the visualization and identification of viral cell tropism in well-differentiated AEC cultures from human, swine, bovine, and feline origin.

Item Type:	Book Section (Book Chapter)
Division/Institute:	09 Interdisciplinary Units > Microscopy Imaging Center (MIC) 04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research 04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases 04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Virology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
Graduate School:	Graduate School for Cellular and Biomedical Sciences (GCB)
UniBE Contributor:	Gultom, Mitra Lovelin, Laloli, Laura, Dijkman, Ronald
Subjects:	600 Technology > 630 Agriculture 500 Science > 570 Life sciences; biology
ISSN:	1940-6029
ISBN:	978-1-0716-0900-2
Series:	Methods in Molecular Biology
Publisher:	Humana, New York, NY
Language:	English
Submitter:	Ronald Dijkman
Date Deposited:	11 Jan 2021 11:01
Last Modified:	15 Mar 2023 15:57
Publisher DOI:	10.1007/978-1-0716-0900-2_10
PubMed ID:	32833209
Uncontrolled Keywords:	Animal airway epithelial cell cultures Cell tropism Veterinary coronavirus Virus characterization
URI:	https://boris.unibe.ch/id/eprint/151144