Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures.

Gultom, Mitra; Laloli, Laura; Dijkman, Ronald (2020). Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures. In: Maier, Helena J.; Bickerton, Erica (eds.) Coronaviruses. Methods and Protocols. Methods in Molecular Biology: Vol. 2203 (pp. 119-134). Humana, New York, NY 10.1007/978-1-0716-0900-2_10

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Well-differentiated primary airway epithelial cell (AEC) cultures have been widely used for the characterization of several human respiratory viruses including coronaviruses. In recent years, there has been an increase in interest toward animal AEC cultures and their application to characterize veterinary viruses with zoonotic potential, as well as studying host-pathogen interactions in animal reservoir host species. In this chapter, we provide a revised and improved protocol for the isolation and establishment of well-differentiated AEC cultures from diverse mammalian species and the use of the cultures for the characterization of veterinary coronavirus. We also describe immunohistochemistry protocols with validated antibodies for the visualization and identification of viral cell tropism in well-differentiated AEC cultures from human, swine, bovine, and feline origin.

Item Type:

Book Section (Book Chapter)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Virology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Gultom, Mitra Lovelin; Laloli, Laura and Dijkman, Ronald

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

1940-6029

ISBN:

978-1-0716-0900-2

Series:

Methods in Molecular Biology

Publisher:

Humana, New York, NY

Language:

English

Submitter:

Ronald Dijkman

Date Deposited:

11 Jan 2021 11:01

Last Modified:

11 Jan 2021 11:01

Publisher DOI:

10.1007/978-1-0716-0900-2_10

PubMed ID:

32833209

Uncontrolled Keywords:

Animal airway epithelial cell cultures Cell tropism Veterinary coronavirus Virus characterization

URI:

https://boris.unibe.ch/id/eprint/151144

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