Mechanical properties of soft biological membranes for organ-on-a-chip assessed by bulge test and AFM

Zamprogno, Pauline; Thoma, Giuditta; Cencen, Veronika; Ferrari, Dario; Putz, Barbara; Michler, Johann; Fantner, Georg E.; Guenat, Olivier T. (2021). Mechanical properties of soft biological membranes for organ-on-a-chip assessed by bulge test and AFM. ACS biomaterials science & engineering, 7(7), pp. 2990-2997. American Chemical Society 10.1021/acsbiomaterials.0c00515

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Advanced in vitro models called "organ-on-a-chip" can mimic the specific cellular environment found in various tissues. Many of these models include a thin, sometimes flexible, membrane aimed at mimicking the extracellular matrix (ECM) scaffold of in vivo barriers. These membranes are often made of polydimethylsiloxane (PDMS), a silicone rubber that poorly mimics the chemical and physical properties of the basal membrane. However, the ECM and its mechanical properties play a key role in the homeostasis of a tissue. Here, we report about biological membranes with a composition and mechanical properties similar to those found in vivo. Two types of collagen-elastin (CE) membranes were produced: vitrified and nonvitrified (called "hydrogel membrane"). Their mechanical properties were characterized using the bulge test method. The results were compared using atomic force microscopy (AFM), a standard technique used to evaluate the Young's modulus of soft materials at the nanoscale. Our results show that CE membranes with stiffnesses ranging from several hundred of kPa down to 1 kPa can be produced by tuning the CE ratio, the production mode (vitrified or not), and/or certain parameters such as temperature. The Young's modulus can easily be determined using the bulge test. This method is a robust and reproducible to determine membrane stiffness, even for soft membranes, which are more difficult to assess by AFM. Assessment of the impact of substrate stiffness on the spread of human fibroblasts on these surfaces showed that cell spread is lower on softer surfaces than on stiffer surfaces.

Item Type:

Journal Article (Original Article)

Division/Institute:

10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Organs-on Chip Technologies

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Zamprogno, Pauline Gil Véronique; Thoma, Giuditta; Ferrari, Dario and Guenat, Olivier Thierry

Subjects:

500 Science > 540 Chemistry
500 Science > 570 Life sciences; biology
600 Technology > 620 Engineering

ISSN:

2373-9878

Publisher:

American Chemical Society

Funders:

[UNSPECIFIED] Swiss National Science Foundation

Language:

English

Submitter:

Olivier Thierry Guenat

Date Deposited:

06 May 2021 08:42

Last Modified:

06 Oct 2021 12:49

Publisher DOI:

10.1021/acsbiomaterials.0c00515

PubMed ID:

33651947

BORIS DOI:

10.48350/152215

URI:

https://boris.unibe.ch/id/eprint/152215

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