Transcriptome of microglia reveals a species-specific expression profile in bovines with conserved and new signature genes.

Tavares-Gomes, Leticia; Monney, Camille; Neuhaus, Géraldine; Francisco, David; Solis, Diana; Summerfield, Artur; Erny, Daniel; Jagannathan, Vidhya; Oevermann, Anna (2021). Transcriptome of microglia reveals a species-specific expression profile in bovines with conserved and new signature genes. GLIA, 69(8), pp. 1932-1949. Wiley-Blackwell 10.1002/glia.24002

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Evidence is growing that microglia adopt different roles than monocyte-derived macrophages (MDM) during CNS injury. However, knowledge about their function in the pathogenesis of neuroinfections is only rudimentary. Cattle are frequently affected by neuroinfections that are either zoonotic or related to diseases in humans, and, hence, studies of bovine neuroinfections as a natural disease model may generate fundamental data on their pathogenesis potentially translatable to humans. We investigated the transcriptomic landscape and lineage markers of bovine microglia and MDM. Although bovine microglia expressed most microglial signature genes known from humans and mice, they exhibited a species-specific transcriptomic profile, including strikingly low expression of TMEM119 and enrichment of the two scavenger receptors MEGF10 and LY75. P2RY12 was amongst the most enriched genes in bovine microglia, and antibodies against P2RY12 labeled specifically resting microglia, but also reactive microglia within neuroinfection foci in-situ. On the other hand, F13A1 was amongst the most enriched genes in bovine monocytes and MDM and, additionally, the encoded protein was expressed in-situ in monocytes and MDM in the inflamed brain but not in microglia, making it a promising marker for infiltrating MDM in the brain. In culture, primary bovine microglia downregulated signature genes, expressed markers of activation, and converged their transcriptome to MDM. However, they retained several microglia signature genes that clearly distinguished them from bovine MDM, making them a promising in-vitro tool to study mechanisms of microglia-pathogen interactions.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Monney, Camille; Neuhaus, Géraldine; Solis, Diana; Summerfield, Artur; Jagannathan, Vidya and Oevermann, Anna

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

0894-1491

Publisher:

Wiley-Blackwell

Funders:

[4] Swiss National Science Foundation ; [24] Gottfried und Julia Bangerter- Rhyner Stiftung ; [UNSPECIFIED] Frauchiger Stiftung

Language:

English

Submitter:

Anna Oevermann

Date Deposited:

26 Apr 2021 12:50

Last Modified:

11 Jun 2021 01:33

Publisher DOI:

10.1002/glia.24002

PubMed ID:

33811399

Uncontrolled Keywords:

F13A1 MDM P2RY12 TMEM119 in-vitro model microglia monocytes

BORIS DOI:

10.48350/155979

URI:

https://boris.unibe.ch/id/eprint/155979

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