In-yeast reconstruction of the African swine fever virus genome isolated from clinical samples.

Labroussaa, Fabien; Mehinagic, Kemal; Cippa, Valentina; Liniger, Matthias; Akarsu, Hatice; Ruggli, Nicolas; Jores, Joerg (2021). In-yeast reconstruction of the African swine fever virus genome isolated from clinical samples. STAR protocols, 2(3), p. 100803. Elsevier 10.1016/j.xpro.2021.100803

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This protocol describes a synthetic genomics pipeline to clone and engineer the entire 190-kbp genome of the African swine fever virus (ASFV) genotype II in yeast using transformation-associated recombination cloning. The viral genome was cloned using DNA directly extracted from a clinical sample. In addition, the precise deletion of a non-essential gene and its replacement by a synthetic reporter gene cassette are presented. This protocol is applicable to other ASFV genotypes and other large DNA viruses.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Labroussaa, Fabien; Cippà, Valentina Lucia; Liniger, Matthias; Akarsu Egger, Hatice; Ruggli, Nicolas and Jores, Jörg

Subjects:

600 Technology > 630 Agriculture

ISSN:

2666-1667

Publisher:

Elsevier

Language:

English

Submitter:

Katharina Gerber-Paizs

Date Deposited:

28 Sep 2021 09:43

Last Modified:

28 Sep 2021 09:43

Publisher DOI:

10.1016/j.xpro.2021.100803

PubMed ID:

34527959

Uncontrolled Keywords:

Biotechnology and bioengineering Genomics Health Sciences Microbiology Molecular Biology Sequencing Systems biology

BORIS DOI:

10.48350/159547

URI:

https://boris.unibe.ch/id/eprint/159547

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