In-yeast reconstruction of the African swine fever virus genome isolated from clinical samples.

Labroussaa, Fabien; Mehinagic, Kemal; Cippa, Valentina; Liniger, Matthias; Akarsu, Hatice; Ruggli, Nicolas; Jores, Joerg (2021). In-yeast reconstruction of the African swine fever virus genome isolated from clinical samples. STAR protocols, 2(3), p. 100803. Elsevier 10.1016/j.xpro.2021.100803

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This protocol describes a synthetic genomics pipeline to clone and engineer the entire 190-kbp genome of the African swine fever virus (ASFV) genotype II in yeast using transformation-associated recombination cloning. The viral genome was cloned using DNA directly extracted from a clinical sample. In addition, the precise deletion of a non-essential gene and its replacement by a synthetic reporter gene cassette are presented. This protocol is applicable to other ASFV genotypes and other large DNA viruses.

Item Type:	Journal Article (Further Contribution)
Division/Institute:	05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
Graduate School:	Graduate School for Cellular and Biomedical Sciences (GCB)
UniBE Contributor:	Labroussaa, Fabien, Mehinagic, Kemal, Cippà, Valentina Lucia, Liniger, Matthias, Akarsu Egger, Hatice, Ruggli, Nicolas, Jores, Jörg
Subjects:	500 Science 500 Science > 570 Life sciences; biology 500 Science > 590 Animals (Zoology) 600 Technology > 610 Medicine & health 600 Technology > 630 Agriculture
ISSN:	2666-1667
Publisher:	Elsevier
Language:	English
Submitter:	Katharina Gerber-Paizs
Date Deposited:	28 Sep 2021 09:43
Last Modified:	05 Dec 2022 15:53
Publisher DOI:	10.1016/j.xpro.2021.100803
PubMed ID:	34527959
Uncontrolled Keywords:	Biotechnology and bioengineering Genomics Health Sciences Microbiology Molecular Biology Sequencing Systems biology
BORIS DOI:	10.48350/159547
URI:	https://boris.unibe.ch/id/eprint/159547

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In-yeast reconstruction of the African swine fever virus genome isolated from clinical samples.

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