Development of safe and highly protective live-attenuated SARS-CoV-2 vaccine candidates by genome recoding.

Trimpert, Jakob; Dietert, Kristina; Firsching, Theresa C; Ebert, Nadine; Tran, Thi Nhu Thao; Vladimirova, Daria; Kaufer, Susanne; Labroussaa, Fabien; Abdelgawad, Azza; Conradie, Andelé; Höfler, Thomas; Adler, Julia M; Bertzbach, Luca D; Jores, Jörg; Gruber, Achim D; Thiel, Volker; Osterrieder, Nikolaus; Kunec, Dusan (2021). Development of safe and highly protective live-attenuated SARS-CoV-2 vaccine candidates by genome recoding. Cell reports, 36(5), p. 109493. Cell Press 10.1016/j.celrep.2021.109493

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Safe and effective vaccines are urgently needed to stop the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We construct a series of live attenuated vaccine candidates by large-scale recoding of the SARS-CoV-2 genome and assess their safety and efficacy in Syrian hamsters. Animals were vaccinated with a single dose of the respective recoded virus and challenged 21 days later. Two of the tested viruses do not cause clinical symptoms but are highly immunogenic and induce strong protective immunity. Attenuated viruses replicate efficiently in the upper but not in the lower airways, causing only mild pulmonary histopathology. After challenge, hamsters develop no signs of disease and rapidly clear challenge virus: at no time could infectious virus be recovered from the lungs of infected animals. The ease with which attenuated virus candidates can be produced and administered favors their further development as vaccines to combat the ongoing pandemic.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Ebert, Nadine; Tran, Thi Nhu Thao; Labroussaa, Fabien; Jores, Jörg and Thiel, Volker Earl

Subjects:

600 Technology > 630 Agriculture

ISSN:

2211-1247

Publisher:

Cell Press

Language:

English

Submitter:

Pamela Schumacher

Date Deposited:

18 Nov 2021 09:55

Last Modified:

18 Nov 2021 09:56

Publisher DOI:

10.1016/j.celrep.2021.109493

PubMed ID:

34320400

Uncontrolled Keywords:

COVID-19 Roborovski dwarf hamster SARS-CoV-2 Syrian hamster codon pair deoptimization coronavirus genome recoding live attenuated vaccine synthetic attenuated virus engineering

BORIS DOI:

10.48350/160716

URI:

https://boris.unibe.ch/id/eprint/160716

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