Genomic analyses of human adenoviruses unravel novel recombinant genotypes associated with severe infections in pediatric patients.

Akello, Joyce Odeke; Kamgang, Richard; Barbani, Maria Teresa; Suter-Riniker, Franziska; Aebi, Christoph; Beuret, Christian; Paris, Daniel H.; Leib, Stephen L.; Ramette, Alban (2021). Genomic analyses of human adenoviruses unravel novel recombinant genotypes associated with severe infections in pediatric patients. Scientific reports, 11(1), p. 24038. Springer Nature 10.1038/s41598-021-03445-y

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Human adenoviruses (HAdVs) are highly contagious pathogens of clinical importance, especially among the pediatric population. Studies on comparative viral genomic analysis of cases associated with severe and mild infections due to HAdV are limited. Using whole-genome sequencing (WGS), we investigated whether there were any differences between circulating HAdV strains associated with severe infections (meningitis, sepsis, convulsion, sudden infant death syndrome, death, and hospitalization) and mild clinical presentations in pediatric patients hospitalized between the years 1998 and 2017 in a tertiary care hospital group in Bern, Switzerland covering a population base of approx. 2 million inhabitants. The HAdV species implicated in causing severe infections in this study included HAdV species C genotypes (HAdV1, HAdV2, and HAdV5). Clustering of the HAdV whole-genome sequences of the severe and mild cases did not show any differences except for one sample (isolated from a patient presenting with sepsis, meningitis, and hospitalization) that formed its own cluster with HAdV species C genotypes. This isolate showed intertypic recombination events involving four genotypes, had the highest homology to HAdV89 at complete genome level, but possessed the fiber gene of HAdV1, thereby representing a novel genotype of HAdV species C. The incidence of potential recombination events was higher in severe cases than in mild cases. Our findings confirm that recombination among HAdVs is important for molecular evolution and emergence of new strains. Therefore, further research on HAdVs, particularly among susceptible groups, is needed and continuous surveillance is required for public health preparedness including outbreak investigations.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Clinical Microbiology
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Infection Serology
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Virology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Akello, Joyce Odeke; Kamgang, Richard; Barbani, Maria Teresa; Suter, Franziska Marta; Aebi, Christoph; Leib, Stephen and Ramette, Alban Nicolas

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2045-2322

Publisher:

Springer Nature

Funders:

[164] Marie Skłodowska-Curie Actions (European Commission)

Language:

English

Submitter:

Stephen Leib

Date Deposited:

27 Dec 2021 07:41

Last Modified:

02 Jan 2022 01:53

Publisher DOI:

10.1038/s41598-021-03445-y

PubMed ID:

34912023

BORIS DOI:

10.48350/162594

URI:

https://boris.unibe.ch/id/eprint/162594

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