Efficient Recovery of Attenuated Canine Distemper Virus from cDNA.

Wyss, Marianne; Gradauskaite, Vaiva; Ebert, Nadine; Thiel, Volker; Zurbriggen, Andreas; Plattet, Philippe (2022). Efficient Recovery of Attenuated Canine Distemper Virus from cDNA. Virus research, 316, p. 198796. Elsevier 10.1016/j.virusres.2022.198796

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To provide insights into the biology of the attenuated canine distemper virus (CDV) Onderstepoort (OP) strain (large plaque forming variant), design next-generation multivalent vaccines, or further investigate its promising potential as an oncolytic vector, we employed contemporary modifications to establish an efficient OP-CDV-based reverse genetics platform. Successful viral rescue was obtained however only upon recovery of a completely conserved charged residue (V13E) residing at the N-terminal region of the large protein (L). Although L-V13 and L-V13E did not display drastic differences in cellular localization and physical interaction with P, efficient polymerase complex (P+L) activity was recorded only with L-V13E. Interestingly, grafting mNeonGreen to the viral N protein via a P2A ribosomal skipping sequence (OPneon) and its derivative V-protein-knockout variant (OPneon-Vko) exhibited delayed replication kinetics in cultured cells. Collectively, we established an efficient OP-CDV-based reverse genetics system that enables the design of various strategies potentially contributing to veterinary medicine and research.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Wyss, Marianne; Gradauskaite, Vaiva; Ebert, Nadine; Thiel, Volker Earl and Plattet, Philippe

Subjects:

600 Technology > 630 Agriculture

ISSN:

1872-7492

Publisher:

Elsevier

Language:

English

Submitter:

Pubmed Import

Date Deposited:

17 May 2022 12:27

Last Modified:

22 May 2022 01:54

Publisher DOI:

10.1016/j.virusres.2022.198796

PubMed ID:

35568090

Uncontrolled Keywords:

Morbillivirus V protein large protein phosphoprotein replication reverse genetics

BORIS DOI:

10.48350/170038

URI:

https://boris.unibe.ch/id/eprint/170038

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