Kronenberg, Philipp A; Deibel, Ansgar; Gottstein, Bruno; Grimm, Felix; Müllhaupt, Beat; Meyer Zu Schwabedissen, Cordula; Aitbaev, Sezdbek; Omorov, Rakhatbek A; Abdykerimov, Kubanychbek K; Minbaeva, Gulnara; Usubalieva, Jumagul; Siles-Lucas, Mar; Pepe, Paola; Rinaldi, Laura; Spiliotis, Markus; Wang, Junhua; Müller, Norbert; Torgerson, Paul R; Deplazes, Peter (2022). Serological Assays for Alveolar and Cystic Echinococcosis-A Comparative Multi-Test Study in Switzerland and Kyrgyzstan. Pathogens, 11(5) MDPI AG 10.3390/pathogens11050518
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Both alveolar (AE) and cystic echinococcosis (CE) are lacking pathognomonic clinical signs; consequently imaging technologies and serology remain the main pillars for diagnosis. The present study included 100 confirmed treatment-naïve AE and 64 CE patients that were diagnosed in Switzerland or Kyrgyzstan. Overall, 10 native Echinococcus spp. antigens, 3 recombinant antigens, and 4 commercial assays were comparatively evaluated. All native E. multilocularis antigens were produced in duplicates with a European and a Kyrgyz isolate and showed identical test values for the diagnosis of AE and CE. Native antigens and three commercial tests showed high diagnostic sensitivities (Se: 86-96%) and specificities (Sp: 96-99%) for the diagnosis of AE and CE in Swiss patients. In Kyrgyz patients, values of sensitivities and specificities were 10-20% lower as compared to the Swiss patients' findings. For the sero-diagnosis of AE in Kyrgyzstan, a test-combination of an E. multilocularis protoscolex antigen and the recombinant antigen Em95 appears to be the most suitable test strategy (Se: 98%, Sp: 87%). For the diagnosis of CE in both countries, test performances were hampered by major cross-reactions with AE patients and other parasitic diseases as well as by limited diagnostic sensitivities (93% in Switzerland and 76% in Kyrgyzstan, respectively).
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research 04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases 04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Parasitology |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Gottstein, Bruno, Wang, Junhua |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
2076-0817 |
Publisher: |
MDPI AG |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
30 May 2022 09:34 |
Last Modified: |
05 Dec 2022 16:20 |
Publisher DOI: |
10.3390/pathogens11050518 |
PubMed ID: |
35631039 |
Uncontrolled Keywords: |
ELISA Echinococcus granulosus sensu lato Echinococcus multilocularis Western blot antibodies antigens diagnosis serology |
BORIS DOI: |
10.48350/170321 |
URI: |
https://boris.unibe.ch/id/eprint/170321 |
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