Investigating Antiprotozoal Chemotherapies with Novel Proteomic Tools-Chances and Limitations: A Critical Review.

Müller, Joachim; Boubaker, Ghalia; Müller, Norbert; Uldry, Anne-Christine; Braga-Lagache, Sophie; Heller, Manfred; Hemphill, Andrew (2024). Investigating Antiprotozoal Chemotherapies with Novel Proteomic Tools-Chances and Limitations: A Critical Review. International journal of molecular sciences, 25(13) MDPI 10.3390/ijms25136903

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Identification of drug targets and biochemical investigations on mechanisms of action are major issues in modern drug development. The present article is a critical review of the classical "one drug"-"one target" paradigm. In fact, novel methods for target deconvolution and for investigation of resistant strains based on protein mass spectrometry have shown that multiple gene products and adaptation mechanisms are involved in the responses of pathogens to xenobiotics rather than one single gene or gene product. Resistance to drugs may be linked to differential expression of other proteins than those interacting with the drug in protein binding studies and result in complex cell physiological adaptation. Consequently, the unraveling of mechanisms of action needs approaches beyond proteomics. This review is focused on protozoan pathogens. The conclusions can, however, be extended to chemotherapies against other pathogens or cancer.

Item Type:

Journal Article (Review Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Massenspektrometrie- und Proteomics-Labor

UniBE Contributor:

Müller, Heinz Joachim, Boubaker, Ghalia, Müller, Norbert, Uldry, Anne-Christine, Braga, Sophie Marie-Pierre, Heller, Manfred, Hemphill, Andrew

Subjects:

600 Technology > 630 Agriculture
600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

1422-0067

Publisher:

MDPI

Language:

English

Submitter:

Pubmed Import

Date Deposited:

16 Jul 2024 14:11

Last Modified:

17 Jul 2024 13:24

Publisher DOI:

10.3390/ijms25136903

PubMed ID:

39000012

Uncontrolled Keywords:

adaptation mass spectrometry mode of action model system resistance target

BORIS DOI:

10.48350/198986

URI:

https://boris.unibe.ch/id/eprint/198986

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