Increase in hippocampal water diffusion and volume during experimental pneumococcal meningitis is aggravated by bacteremia

Holler, Jon G.; Brandt, Christian T.; Leib, Stephen L.; Rowland, Ian J.; Ostergaard, Christian (2014). Increase in hippocampal water diffusion and volume during experimental pneumococcal meningitis is aggravated by bacteremia. BMC infectious diseases, 14(1), p. 240. BioMed Central 10.1186/1471-2334-14-240

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BACKGROUND

The hippocampus undergoes apoptosis in experimental pneumococcal meningitis leading to neurofunctional deficits in learning and memory function. The aim of the present study was 1) to investigate hippocampal apparent diffusion coefficient (ADC) and volume with MRI during the course of experimental pneumococcal meningitis, 2) to explore the influence of accompanying bacteremia on hippocampal water distribution and volume, 3) and to correlate these findings to the extent of apoptosis in the hippocampus.

METHODS

Experimental meningitis in rats was induced by intracisternal injection of live pneumococci. The study comprised of four experimental groups. I. Uninfected controls (n = 8); II. Meningitis (n = 11); III. Meningitis with early onset bacteremia by additional i.v. injection of live pneumococci (n = 10); IV. Meningitis with attenuated bacteremia by treatment with serotype-specific anti-pneumococcal antibodies (n = 14). T2 and diffusion weighted MR images were used to analyze changes in hippocampus volume and water diffusion (ADC). The results were correlated to ADC of the cortex, to ventricular volume, and to the extent of hippocampal apoptosis.

RESULTS

Both ADC and the volume of hippocampus were significantly increased in meningitis rats compared to uninfected controls (Kruskal-Wallis test, p = 0.0001, Dunns Post Test, p < 0.05), and were significantly increased in meningitis rats with an early onset bacteremia as compared to meningitis rats with attenuated bacteremia (p < 0.05). Hippocampal ADC and the volume and size of brain ventricles were positively correlated (Spearman Rank, p < 0.05), whereas no association was found between ADC or volume and the extent of apoptosis (p > 0.05).

CONCLUSIONS

In experimental meningitis increase in volume and water diffusion of the hippocampus are significantly associated with accompanying bacteremia.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research

UniBE Contributor:

Leib, Stephen

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1471-2334

Publisher:

BioMed Central

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Stephen Leib

Date Deposited:

01 Sep 2014 09:48

Last Modified:

28 Nov 2020 02:24

Publisher DOI:

10.1186/1471-2334-14-240

PubMed ID:

24886045

Uncontrolled Keywords:

Magnetic resonance imaging, ADC, Volume, Oedema, Meningitis, Apoptosis, Hippocampus

BORIS DOI:

10.7892/boris.53537

URI:

https://boris.unibe.ch/id/eprint/53537

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