Dynamics of the nasal microbiota in infancy: A prospective cohort study.

Mika, Moana; Mack, Ines; Korten, Insa Christina Severine; Qi, Weihong; Aebi, Susanne; Frey, Urs Peter; Latzin, Philipp; Hilty, Markus (2015). Dynamics of the nasal microbiota in infancy: A prospective cohort study. Journal of allergy and clinical immunology, 135(4), 905-912.e11. Mosby 10.1016/j.jaci.2014.12.1909

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BACKGROUND Understanding the composition and dynamics of the upper respiratory tract microbiota in healthy infants is a prerequisite to investigate the role of the microbiota in patients with respiratory diseases. This is especially true in early life, when the immune system is in development. OBJECTIVE We sought to describe the dynamics of the upper respiratory tract microbiota in healthy infants within the first year of life. METHODS After exclusion of low-quality samples, microbiota characterization was performed by using 16S rDNA pyrosequencing of 872 nasal swabs collected biweekly from 47 unselected infants. RESULTS Bacterial density increased and diversity decreased within the first year of life (R(2) = 0.95 and 0.73, respectively). A distinct profile for the first 3 months of life was found with increased relative abundances of Staphlyococcaceae and Corynebacteriaceae (exponential decay: R(2) = 0.94 and 0.96, respectively). In addition, relative bacterial abundance and composition differed significantly from summer to winter months. The individual composition of the microbiota changed with increasing time intervals between samples and was best modeled by an exponential function (R(2) = 0.97). Within-subject dissimilarity in a 2-week time interval was consistently lower than that between subjects, indicating a personalized microbiota. CONCLUSION This study reveals age and seasonality as major factors driving the composition of the nasal microbiota within the first year of life. A subject's microbiota is personalized but dynamic throughout the first year. These data are indispensable to interpretation of cross-sectional studies and investigation of the role of the microbiota in both healthy subjects and patients with respiratory diseases. They might also serve as a baseline for future intervention studies.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Infectiology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Mika, Moana; Mack, Ines; Korten, Insa Christina Severine; Aebi, Susanne; Frey, Urs Peter; Latzin, Philipp and Hilty, Markus

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0091-6749

Publisher:

Mosby

Language:

English

Submitter:

Annelies Luginbühl

Date Deposited:

10 Feb 2015 10:14

Last Modified:

09 Feb 2017 14:30

Publisher DOI:

10.1016/j.jaci.2014.12.1909

PubMed ID:

25636948

Uncontrolled Keywords:

Nasal microbiota, age, bacterial families, cohort study, season, toddlers

BORIS DOI:

10.7892/boris.62766

URI:

https://boris.unibe.ch/id/eprint/62766

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