Purification, cDNA structure and biological significance of a single insulin-like growth factor-binding domain protein (SIBD-1) identified in the hemocytes of the spider Cupiennius salei

Kuhn-Nentwig, Lucia; Largiadèr, Carlo R; Streitberger, Kathrin; Chandru, Sathyan; Baumann, Tommy; Kämpfer, Urs; Schaller, Johann; Schürch, Stefan; Nentwig, Wolfgang (2011). Purification, cDNA structure and biological significance of a single insulin-like growth factor-binding domain protein (SIBD-1) identified in the hemocytes of the spider Cupiennius salei. Insect biochemistry and molecular biology, 41(11), pp. 891-901. Amsterdam: Elsevier 10.1016/j.ibmb.2011.08.003

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Cupiennius salei single insulin-like growth factor-binding domain protein (SIBD-1), which exhibits an IGFBP N-terminal domain-like profile, was identified in the hemocytes of the spider C. salei. SIBD-1 was purified by RP-HPLC and the sequence determined by a combination of Edman degradation and 5'-3'- RACE PCR. The peptide (8676.08 Da) is composed of 78 amino acids, contains six intrachain disulphide bridges and carries a modified Thr residue at position 2. SIBD-1 mRNA expression was detected by quantitative real-time PCR mainly in hemocytes, but also in the subesophageal nerve mass and muscle. After infection, the SIBD-1 content in the hemocytes decreases and, simultaneously, the temporal SIBD-1 expression seems to be down-regulated. Two further peptides, SIBD-2 and IGFBP-rP1, also exhibiting IGFBP N-terminal domain variants with unknown functions, were identified on cDNA level in spider hemocytes and venom glands. We conclude that SIBD-1 may play an important role in the immune system of spiders.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Community Ecology
04 Faculty of Medicine > Service Sector > Institute of Pathology
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry
08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Kuhn-Nentwig, Lucia; Largiadèr, Carlo Rodolfo; Streitberger, Kathrin; Baumann, Tommy; Kämpfer, Urs; Schaller, Johann; Schürch, Stefan and Nentwig, Wolfgang

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)
500 Science > 580 Plants (Botany)
600 Technology > 610 Medicine & health

ISSN:

0965-1748

Publisher:

Elsevier

Language:

English

Submitter:

Tommy Baumann

Date Deposited:

04 Oct 2013 14:22

Last Modified:

27 Nov 2015 12:26

Publisher DOI:

10.1016/j.ibmb.2011.08.003

PubMed ID:

21888974

Web of Science ID:

000296174200006

URI:

https://boris.unibe.ch/id/eprint/7578 (FactScience: 212872)

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