Streptococcus pneumoniae capsule determines disease severity in experimental pneumococcal meningitis.

Hathaway, Lucy Jane; Grandgirard, Denis; Valente, Luca Gabriele; Täuber, Martin G.; Leib, Stephen (2016). Streptococcus pneumoniae capsule determines disease severity in experimental pneumococcal meningitis. Open Biology, 6(3) The Royal Society Publishing 10.1098/rsob.150269

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Streptococcus pneumoniaebacteria can be characterized into over 90 serotypes according to the composition of their polysaccharide capsules. Some serotypes are common in nasopharyngeal carriage whereas others are associated with invasive disease, but when carriage serotypes do invade disease is often particularly severe. It is unknown whether disease severity is due directly to the capsule type or to other virulence factors. Here, we used a clinical pneumococcal isolate and its capsule-switch mutants to determine the effect of capsule, in isolation from the genetic background, on severity of meningitis in an infant rat model. We found that possession of a capsule was essential for causing meningitis. Serotype 6B caused significantly more mortality than 7F and this correlated with increased capsule thickness in the cerebrospinal fluid (CSF), a stronger inflammatory cytokine response in the CSF and ultimately more cortical brain damage. We conclude that capsule type has a direct effect on meningitis severity. This is an important consideration in the current era of vaccination targeting a subset of capsule types that causes serotype replacement.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Hathaway, Lucy Jane; Grandgirard, Denis; Valente, Luca Gabriele; Täuber, Martin G. and Leib, Stephen

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2046-2441

Publisher:

The Royal Society Publishing

Funders:

Organisations 162583 not found.
[4] Swiss National Science Foundation
[24] Gottfried und Julia Bangerter- Rhyner Stiftung

Language:

English

Submitter:

Lucy Jane Hathaway

Date Deposited:

02 May 2016 16:23

Last Modified:

08 Feb 2017 20:14

Publisher DOI:

10.1098/rsob.150269

PubMed ID:

27009189

BORIS DOI:

10.7892/boris.81021

URI:

https://boris.unibe.ch/id/eprint/81021

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