The nuclear RNA binding protein RBP33 influences mRNA and spliced leader RNA abundance in Trypanosoma brucei

Trikin, Roman; Hoffmann, Anneliese; Doiron, Nicholas; Jakob, Martin; Ochsenreiter, Torsten; Cirovic, Olivera (2017). The nuclear RNA binding protein RBP33 influences mRNA and spliced leader RNA abundance in Trypanosoma brucei. Molecular and biochemical parasitology, 212, pp. 16-20. Elsevier 10.1016/j.molbiopara.2016.12.008

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RNA recognition motif (RRM) containing proteins are important regulators of gene expression in trypanosomes. Here we expand our current knowledge on the exclusively nuclear localized RRM domain containing protein RBP33 of Trypanosoma brucei. Overexpression of RBP33 leads to a quick growth arrest in G2/M in bloodstream form cells likely due to an overall mRNA- and spliced leader abundance decrease while the ribosomal RNAs remain unaffected. The recombinant RBP33 binds to poly(A) and random sequence RNA in vitro confirming its role as a RNA binding protein. Finally super-resolution microscopy detects RBP33 in small punctae throughout the nucleus and surrounding the nucleolus, however the signal is depleted inside the nucleolus.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Trikin, Roman; Hoffmann, Anneliese; Doiron, Nicholas; Jakob, Martin and Ochsenreiter, Torsten

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0166-6851

Publisher:

Elsevier

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Torsten Ochsenreiter

Date Deposited:

23 Jan 2017 14:24

Last Modified:

23 Jan 2017 14:24

Publisher DOI:

10.1016/j.molbiopara.2016.12.008

PubMed ID:

28069429

BORIS DOI:

10.7892/boris.93447

URI:

https://boris.unibe.ch/id/eprint/93447

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