Uchimura, Yasuhiro; Fuhrer, Tobias; Li, Hai; Lawson, Melissa A.; Zimmermann, Michael; Yilmaz, Bahtiyar; Zindel, Joel; Ronchi, Francesca; Sorribas Olivera, Marcel; Hapfelmeier, Siegfried; Ganal-Vonarburg, Stephanie C.; Gomez de Agüero, Mercedes; McCoy, Kathleen D.; Sauer, Uwe; Macpherson, Andrew J. (2018). Antibodies Set Boundaries Limiting Microbial Metabolite Penetration and the Resultant Mammalian Host Response. Immunity, 49(3), 545-559.e5. Cell Press 10.1016/j.immuni.2018.08.004
|
Text
1-s2.0-S1074761318303443-main.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND). Download (5MB) | Preview |
Although the mammalian microbiota is well contained within the intestine, it profoundly shapes development and metabolism of almost every host organ. We questioned the range and depth of microbial metabolite penetration into the host, and how this is modulated by intestinal immunity. Chemically identical microbial and host metabolites were distinguished by stable isotope tracing from C-labeled live non-replicating Escherichia coli, differentiating C host isotopes with high-resolution mass spectrometry. Hundreds of endogenous microbial compounds penetrated 23 host tissues and fluids after intestinal exposure: subsequent C host metabolome signatures included lipidemia, reduced glycolysis, and inflammation. Penetrant bacterial metabolites from the small intestine were rapidly cleared into the urine, whereas induced antibodies curtailed microbial metabolite exposure by accelerating intestinal bacterial transit into the colon where metabolite transport mechanisms are limiting. Pervasive penetration of microbial molecules can cause extensive host tissue responses: these are limited by immune and non-immune intestinal mucosal adaptations to the microbiota.
Actions (login required)
 |
Edit item |