Glycan-specific IgG anti-IgE autoantibodies are protective against allergic anaphylaxis in a murine model.

Engeroff, Paul; Plattner, Kevin; Storni, Federico; Storni, Federico; Thoms, Franziska; Frias Boligan, Kayluz; Mürner, Lukas; Eggel, Alexander; von Gunten, Stephan; Bachmann, Martin F.; Vogel, Monique (2021). Glycan-specific IgG anti-IgE autoantibodies are protective against allergic anaphylaxis in a murine model. Journal of allergy and clinical immunology, 147(4), pp. 1430-1441. Elsevier 10.1016/j.jaci.2020.11.031

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BACKGROUND

IgE causes anaphylaxis in type-1 hypersensitivity diseases by activating degranulation of effector cells such as mast cells and basophils. The mechanisms that control IgE activity and prevent anaphylaxis under normal conditions are still enigmatic.

OBJECTIVE

We aimed to unravel how anti-IgE autoantibodies are induced and understand their role in regulating serum IgE level and allergic anaphylaxis.

METHODS

We immunized mice with different forms of IgE and tested anti-IgE autoantibody responses and their specificities. We then analysed the effect of those antibodies on serum kinetics and their in vitro and in vivo impact on anaphylaxis. Finally, we investigated anti-IgE autoantibodies in human sera.

RESULTS

Immunization of mice with IgE-immune complexes induced glycan-specific anti-IgE autoantibodies. The anti-IgE autoantibodies prevented effector cell sensitization, reduced total IgE serum levels, protected mice from passive and active IgE sensitization, and resulted in cross-protection against different allergens. Furthermore, glycan-specific anti-IgE autoantibodies were present in sera from allergic and non-allergic subjects.

CONCLUSION

In conclusion, we provide first evidence that in the murine model the serum level and anaphylactic activity of IgE may be down-regulated by glycan-specific IgG anti-IgE autoantibodies.

Item Type:

 Journal Article (Original Article)

Division/Institute:

 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Rheumatologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Rheumatologie
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute for Immunology [discontinued]
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology

Graduate School:

 Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

 Engeroff, Paul Simon, Plattner, Kevin, Storni, Federico Lorenzo, Storni, Federico Lorenzo, Frias Boligan, Kayluz, Mürner, Lukas Dominic, Eggel, Alexander, von Gunten, Stephan, Bachmann, Martin (B), Vogel, Monique

Subjects:

 600 Technology > 610 Medicine & health

ISSN:

 1097-6825

Publisher:

 Elsevier

Language:

 English

Submitter:

 Celine Joray

Date Deposited:

 04 Jan 2021 16:23

Last Modified:

 29 Mar 2023 23:37

Publisher DOI:

 10.1016/j.jaci.2020.11.031

PubMed ID:

 33309740

Uncontrolled Keywords:

 Anti-IgE autoantibodies IgE regulation allergy glycans hypersensitivity

BORIS DOI:

 10.48350/149754

URI:

 https://boris.unibe.ch/id/eprint/149754

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