Contu, Lara; Balistreri, Giuseppe; Domanski, Michal; Uldry, Anne-Christine; Mühlemann, Oliver (2021). Characterisation of the Semliki Forest Virus-host cell interactome reveals the viral capsid protein as an inhibitor of nonsense-mediated mRNA decay. PLoS pathogens, 17(5), e1009603. Public Library of Science 10.1371/journal.ppat.1009603
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The positive-sense, single-stranded RNA alphaviruses pose a potential epidemic threat. Understanding the complex interactions between the viral and the host cell proteins is crucial for elucidating the mechanisms underlying successful virus replication strategies and for developing specific antiviral interventions. Here we present the first comprehensive protein-protein interaction map between the proteins of Semliki Forest Virus (SFV), a mosquito-borne member of the alphaviruses, and host cell proteins. Among the many identified cellular interactors of SFV proteins, the enrichment of factors involved in translation and nonsense-mediated mRNA decay (NMD) was striking, reflecting the virus’ hijacking of the translation machinery and indicating viral countermeasures for escaping NMD by inhibiting NMD at later time points during the infectious cycle. In addition to observing a general inhibition of NMD about 4 hours post infection, we also demonstrate that transient expression of the SFV capsid protein is sufficient to inhibit NMD in cells, suggesting that the massive production of capsid protein during the SFV reproduction cycle is responsible for NMD inhibition.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Massenspektrometrie- und Proteomics-Labor |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Contu, Lara, Domanski, Michal, Uldry, Anne-Christine, Mühlemann, Oliver |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISSN: |
1553-7366 |
Publisher: |
Public Library of Science |
Language: |
English |
Submitter: |
Christina Schüpbach |
Date Deposited: |
07 Jun 2021 16:02 |
Last Modified: |
05 Dec 2022 15:51 |
Publisher DOI: |
10.1371/journal.ppat.1009603 |
PubMed ID: |
34019569 |
BORIS DOI: |
10.48350/156725 |
URI: |
https://boris.unibe.ch/id/eprint/156725 |
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