Secretion of human meprin from intestinal epithelial cells depends on differential expression of the alpha and beta subunits.

Lottaz, Daniel; Hahn, Dagmar; Müller, Stefan; Müller, Christoph; Sterchi, Erwin E (1999). Secretion of human meprin from intestinal epithelial cells depends on differential expression of the alpha and beta subunits. European journal of biochemistry, 259(1-2), pp. 496-504. FEBS Press 10.1046/j.1432-1327.1999.00071.x

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Human meprin (N-benzoyl-l-tyrosyl-p-aminobenzoic acid hydrolase, EC 3.4.24.18), an astacin-type metalloprotease, is expressed by intestinal epithelial cells as a dimeric protein complex of alpha and beta subunits. In transfected cells, intracellular proteolytic removal of the membrane anchor from the alpha subunit results in its secretion, while the beta subunit and alpha/beta heterodimers are retained at the cell membrane. We investigated the consequence of differential intracellular processing of alpha and beta subunits in the human small and large intestine using subunit-specific immunohistochemistry, in situ hybridization and biosynthetic studies in organ culture. In the ileum, both subunits localize to the brush-border membrane of villus enterocytes. In contrast, the beta subunit is not expressed in the colon, which leads to the secretion of the alpha subunit. We conclude that differential expression of meprin alpha and beta subunits is a unique means of targeting the proteolytic activity of the alpha subunit either to the brush-border membrane in the ileum or to the lumen in the colon, suggesting dual functions of cell-associated and luminal meprin. Meprin alpha and beta subunits are also coexpressed in distinct lamina propria leukocytes, suggesting an additional role for this protease in leukocyte function in the intestinal mucosa.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Lottaz, Daniel, Müller, Christoph (C), Sterchi, Erwin-Ernst

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0014-2956

Publisher:

FEBS Press

Language:

English

Submitter:

Christoph Müller

Date Deposited:

19 Sep 2022 14:20

Last Modified:

29 Mar 2023 23:38

Publisher DOI:

10.1046/j.1432-1327.1999.00071.x

PubMed ID:

9914532

BORIS DOI:

10.48350/172971

URI:

https://boris.unibe.ch/id/eprint/172971

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