Sudatip, Duangdao; Mostacci, Nadezda; Tiengrim, Surapee; Thamlikitkul, Visanu; Chasiri, Kittipong; Kritiyakan, Anamika; Phanprasit, Wantanee; Thinphovong, Chuanphot; Abdallah, Rim; Baron, Sophie Alexandra; Rolain, Jean-Marc; Morand, Serge; Oppliger, Anne; Hilty, Markus (2023). The risk of pig and chicken farming for carriage and transmission of Escherichia coli containing extended-spectrum beta-lactamase (ESBL) and mobile colistin resistance (mcr) genes in Thailand. Microbial genomics, 9(3) Microbiology Society 10.1099/mgen.0.000951
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South-East Asian countries report a high prevalence of extended-spectrum cephalosporin- (ESC-) and colistin-resistant Escherichia coli (Col-R-Ec). However, there are still few studies describing the molecular mechanisms and transmission dynamics of ESC-R-Ec and, especially, Col-R-Ec. This study aimed to evaluate the prevalence and transmission dynamics of Ec containing extended spectrum beta-lactamases (ESBL) and mobile colistin resistance (mcr) genes using a 'One Health' design in Thailand. The ESC-R-Ec and Col-R-Ec isolates of human stool samples (69 pig farmers, 155 chicken farmers, and 61 non-farmers), rectal swabs from animals (269 pigs and 318 chickens), and the intestinal contents of 196 rodents were investigated. Resistance mechanisms and transmission dynamics of Ec isolates (n=638) were studied using short and long read sequencing. We found higher rates of ESBL-Ec isolates among pig farmers (n=36; 52.2%) than among chicken farmers (n=58; 37.4 %; P<0.05) and the control group (n=61; 31.1 %; P<0.05). Ec with co-occurring ESBL and mcr genes were found in 17 (6.0 %), 50 (18.6 %) and 15 (4.7 %) samples from humans, pigs and chickens, respectively. We also identified 39 (13.7 %) human samples with non-identical Ec containing ESBL and mcr. We found higher rates of ESBL-Ec, in particular CTX-M-55, isolates among pig farmers than among non-pig farmers (P<0.01). 'Clonal' animal-human transmission of ESBL-Ec and Ec with mcr genes was identified but rare as we overall found a heterogenous population structure of Ec. The Col-R-Ec from human and animal samples often carried mcr-1.1 on conjugative IncX4 plasmids. The latter has been identified in Ec of many different clonal backgrounds.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research 04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases |
UniBE Contributor: |
Sudatip, Duangdao, Mostacci, Nadezda, Hilty, Markus |
Subjects: |
600 Technology > 610 Medicine & health 500 Science > 570 Life sciences; biology |
ISSN: |
2057-5858 |
Publisher: |
Microbiology Society |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
24 Mar 2023 15:59 |
Last Modified: |
24 Mar 2023 23:27 |
Publisher DOI: |
10.1099/mgen.0.000951 |
PubMed ID: |
36951912 |
Uncontrolled Keywords: |
Extended-spectrum beta-lactamase One Health clonal transmission mobile colistin resistance occupational exposure pig farm |
BORIS DOI: |
10.48350/180609 |
URI: |
https://boris.unibe.ch/id/eprint/180609 |
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