Papapostolou, Irida; Bochen, Florian; Peinelt, Christine; Maldifassi, Maria Constanza (2023). A Simple and Fast Method for the Formation and Downstream Processing of Cancer-Cell-Derived 3D Spheroids: An Example Using Nicotine-Treated A549 Lung Cancer 3D Spheres. Methods and protocols, 6(5) MDPI AG 10.3390/mps6050094
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Although 2D in vitro cancer cell cultures have been used for decades as a first line-of-research tool to investigate antitumoral drugs and treatments, their use presents many drawbacks, including the poor resemblance of such cultures to the characteristics of in vivo tumors. To mitigate these drawbacks, 3D culture models have emerged as a more representative alternative. Cancer cells cultured as 3D structures have the advantage of resembling solid tumors in their architecture and in their resistance to chemotherapeutic drugs, in part because of restrained drug penetration. Additionally, these 3D structures create a more physiological environment for the study of immune cell invasion and migration, comparable to solid tumors. In this paper, we describe a fast and cost-effective step-by-step protocol for the generation of 3D spheres using ultra-low-attachment (ULA) multiwell plates, which can be incorporated into the normal workflow of any laboratory. Using this protocol, spheroids of different human cancer cell lines can be obtained and can then be characterized on the basis of their morphology, viability, and expression of specific markers.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Papapostolou, Irida, Bochen, Florian Martin, Peinelt, Christine, Maldifassi, Maria Constanza |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
2409-9279 |
Publisher: |
MDPI AG |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
30 Oct 2023 12:31 |
Last Modified: |
09 Nov 2023 14:05 |
Publisher DOI: |
10.3390/mps6050094 |
PubMed ID: |
37888026 |
Uncontrolled Keywords: |
3D cell culture cancer cells low-attachment surface nicotine nicotinic acetylcholine receptors spheroids three-dimensional cell culture |
BORIS DOI: |
10.48350/188255 |
URI: |
https://boris.unibe.ch/id/eprint/188255 |
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