Schimanski, Bernd; Kräuchi, Rahel; Stettler, Jolanda; Lejon Crottet, Sofia; Niederhauser, Christoph; Clausen, Frederik Banch; Fontana, Stefano; Hodel, Markus; Amylidi-Mohr, Sofia; Raio, Luigi; Abbal, Claire; Henny, Christine (2023). Fetal RHD Screening in RH1 Negative Pregnant Women: Experience in Switzerland. Biomedicines, 11(10) MDPI 10.3390/biomedicines11102646
|
Text
biomedicines-11-02646.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (591kB) | Preview |
RH1 incompatibility between mother and fetus can cause hemolytic disease of the fetus and newborn. In Switzerland, fetal RHD genotyping from maternal blood has been recommended from gestational age 18 onwards since the year 2020. This facilitates tailored administration of RH immunoglobulin (RHIG) only to RH1 negative women carrying a RH1 positive fetus. Data from 30 months of noninvasive fetal RHD screening is presented. Cell-free DNA was extracted from 7192 plasma samples using a commercial kit, followed by an in-house qPCR to detect RHD exons 5 and 7, in addition to an amplification control. Valid results were obtained from 7072 samples, with 4515 (64%) fetuses typed RHD positive and 2556 (36%) fetuses being RHD negative. A total of 120 samples led to inconclusive results due to the presence of maternal or fetal RHD variants (46%), followed by women being serologically RH1 positive (37%), and technical issues (17%). One sample was typed false positive, possibly due to contamination. No false negative results were observed. We show that unnecessary administration of RHIG can be avoided for more than one third of RH1 negative pregnant women in Switzerland. This reduces the risks of exposure to a blood-derived product and conserves this limited resource to women in actual need.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research 04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases 04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology |
UniBE Contributor: |
Niederhauser-Lüthi, Christoph Peter, Amylidi-Mohr, Sofia, Raio, Luigi |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2227-9059 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
30 Oct 2023 12:40 |
Last Modified: |
30 Oct 2023 12:48 |
Publisher DOI: |
10.3390/biomedicines11102646 |
PubMed ID: |
37893020 |
Uncontrolled Keywords: |
RH immunoglobulin prophylaxis RH1 incompatibility cell-free fetal DNA hemolytic disease of the fetus and newborn (HDFN) noninvasive prenatal diagnosis |
BORIS DOI: |
10.48350/188269 |
URI: |
https://boris.unibe.ch/id/eprint/188269 |
Actions (login required)
 |
Edit item |