Hosseini, Aref; Germic, Nina; Markov, Nikita; Stojkov, Darko; Oberson, Kevin; Yousefi, Shida; Simon, Hans-Uwe (2023). The regulatory role of eosinophils in adipose tissue depends on autophagy. Frontiers in immunology, 14, p. 1331151. Frontiers Research Foundation 10.3389/fimmu.2023.1331151
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INTRODUCTION
Obesity is a metabolic condition that elevates the risk of all-cause mortality. Brown and beige adipose tissues, known for their thermogenic properties, offer potential therapeutic targets for combating obesity. Recent reports highlight the role of immune cells, including eosinophils, in adipose tissue homeostasis, while the underlying mechanisms are poorly understood.
METHODS
To study the role of autophagy in eosinophils in this process, we used a genetic mouse model lacking autophagy-associated protein 5 (Atg5), specifically within the eosinophil lineage (Atg5 eoΔ).
RESULTS
The absence of Atg5 in eosinophils led to increased body weight, impaired glucose metabolism, and alterations in the cellular architecture of adipose tissue. Our findings indicate that Atg5 modulates the functional activity of eosinophils within adipose tissue rather than their abundance. Moreover, RNA-seq analysis revealed upregulation of arginase 2 (Arg2) in Atg5-knockout eosinophils. Increased Arg2 activity was shown to suppress adipocyte beiging. Furthermore, we observed enrichment of the purine pathway in the absence of Atg5 in eosinophils, leading to a pro-inflammatory shift in macrophages and a further reduction in beiging.
DISCUSSION
The data shed light on the importance of autophagy in eosinophils and its impact on adipose tissue homeostasis by suppressing Arg2 expression and limiting inflammation in adipose tissue.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
09 Interdisciplinary Units > Microscopy Imaging Center (MIC) 04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Pharmacology |
UniBE Contributor: |
Hosseini, Aref, Germic, Nina, Markov, Nikita, Stojkov, Darko, Oberson, Kevin, Yousefi, Shida, Simon, Hans-Uwe |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1664-3224 |
Publisher: |
Frontiers Research Foundation |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
22 Jan 2024 14:35 |
Last Modified: |
20 Sep 2024 08:49 |
Publisher DOI: |
10.3389/fimmu.2023.1331151 |
PubMed ID: |
38235134 |
Uncontrolled Keywords: |
adipose tissue Atg5 arginase 2 autophagy beiging eosinophils macrophages |
BORIS DOI: |
10.48350/191773 |
URI: |
https://boris.unibe.ch/id/eprint/191773 |
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