Bermudez-Lekerika, Paola; Crump, Katherine B; Wuertz-Kozak, Karin; Le Maitre, Christine L; Gantenbein, Benjamin (2024). Sulfated Hydrogels as Primary Intervertebral Disc Cell Culture Systems. Gels, 10(5) MDPI 10.3390/gels10050330
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The negatively charged extracellular matrix plays a vital role in intervertebral disc tissues, providing specific cues for cell maintenance and tissue hydration. Unfortunately, suitable biomimetics for intervertebral disc regeneration are lacking. Here, sulfated alginate was investigated as a 3D culture material due to its similarity to the charged matrix of the intervertebral disc. Precursor solutions of standard alginate, or alginate with 0.1% or 0.2% degrees of sulfation, were mixed with primary human nucleus pulposus cells, cast, and cultured for 14 days. A 0.2% degree of sulfation resulted in significantly decreased cell density and viability after 7 days of culture. Furthermore, a sulfation-dependent decrease in DNA content and metabolic activity was evident after 14 days. Interestingly, no significant differences in cell density and viability were observed between surface and core regions for sulfated alginate, unlike in standard alginate, where the cell number was significantly higher in the core than in the surface region. Due to low cell numbers, phenotypic evaluation was not achieved in sulfated alginate biomaterial. Overall, standard alginate supported human NP cell growth and viability superior to sulfated alginate; however, future research on phenotypic properties is required to decipher the biological properties of sulfated alginate in intervertebral disc cells.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) |
Graduate School: |
Graduate School for Cellular and Biomedical Sciences (GCB) |
UniBE Contributor: |
Bermudez, Paola, Crump, Katherine Briana, Gantenbein, Benjamin |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2310-2861 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Pubmed Import |
Date Deposited: |
29 May 2024 14:51 |
Last Modified: |
30 May 2024 11:23 |
Publisher DOI: |
10.3390/gels10050330 |
PubMed ID: |
38786247 |
Uncontrolled Keywords: |
3D culture cell viability intervertebral disc qPCR sulfation alginate |
BORIS DOI: |
10.48350/197072 |
URI: |
https://boris.unibe.ch/id/eprint/197072 |
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