Bernasconi, Odette Joëlle; Donà, Valentina; Tinguely, Regula; Endimiani, Andrea (2018). In Vitro Activity of 3 Commercial Bacteriophage Cocktails Against Salmonella and Shigella spp. Isolates of Human Origin. Pathogens and Immunity, 3(1), pp. 72-81. Case Western Reserve University, Cleveland, OH 10.20411/pai.v3i1.234
|
Text (Final version published)
Bernasconi Final 2018.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (228kB) | Preview |
Background: Salmonella and Shigella spp. are two of the most frequent and deadly enteric bacterial pathogens recorded worldwide. In developing countries, Salmonella infections are responsible for many deaths annually and these mortality rates are prone to increase due to the emergence of resistance to antibiotics. In this overall scenario, new alternative therapeutic approaches are needed.
Methods: For the first time, we investigated the activity of three commercial bacteriophage cocktails (INTESTI, Septaphage, PYO) against a collection of contemporary Salmonella spp. (n=30) and Shigella spp. (n=20) strains isolated in Switzerland. Phage susceptibility was determined by implementing the spot test.
Results: The overall susceptibility of Salmonella spp. to INTESTI and Septaphage was 87% and 77%, respectively. With regard to Shigella spp., the overall susceptibility to INTESTI and Septaphage was 95% and 55%, respectively. PYO showed to be active against only 10% of Salmonella spp. but to 95% of Shigella spp.
Conclusions: Our results seem promising, especially for the INTESTI biopreparation against Salmonella enterica infections. Nevertheless, such speculation should be supported by further in vivo studies to confirm its efficacy and to prove its safety. We also emphasize the importance of large in vitro screening analyses aimed to assess the activity of such biopreparations against contemporary multidrug-resistant strains that are emerging worldwide.
Actions (login required)
 |
Edit item |